乳酸杆菌的分离、鉴定及体外拮抗试验.pdfVIP

乳酸杆菌的 鉴定及体外拮抗试验 芦 (四川省雅安职业技术学院药学检验系，四川 雅安 625000) 中图分类号 ：$852．615 文献标识码 ：B 文章编号：1001—8964(2011)11—0024一O3 摘要：本研究利用不同配方的培养基选择性分离、纯化不同生长营养需求的菌种，然后对所分离茵 株作形态观察、生理生化鉴定．再用牛津杯法进行乳酸杆菌全茵液与上清液对大肠杆菌拮抗作用的对 比 试验。结果分别从鸡粪、牛粪、猪粪中分离、纯化出发酵乳杆菌、发状乳杆茵、木糖乳杆茵；乳酸杆菌培养 上清液的拮抗作用大于全茵液；鸡源发酵乳杆菌对猪源大肠杆茵有较好的生物拮抗作用：猪源木糖乳杆 茵及牛源发状乳杆菌对猪源大肠杆菌无生物拮抗作用 关键词：乳酸杆茵：生物拮抗作用：牛津杯 Isolationand identification oflactobacillusand the biologicalantagonisticaction in vitro LU Ke · (PharmaceuticalandInspectionDepartmentofYaanVocationalandTechnologicalCollege SichuanYaan625000，China) Abstract：In this paper，we used culture mediums ofdifferentformula to isolate and purify the microbial strainswitlldifferentgrowth nutrition demand．and classified these microbial strainsthatwe had isolated，by the means ofthe shape observation，the physiological biochemistry reaction．Then， with the use ofoxford-cup，we made a contrastexperimentbetween lactobacillusbacterialfluid nad superior-clear liquid which had the antagonistic action to E．coli．The result showed thatwe had isolated nad purified lactobacillus femr entum， lactobacillus trichoctes nad lactobacillus xylosus from chicken manure， COW dung， pig excrement；the antagonistic action to E．coliof the lactobacillus superior-clear liquid Was strongerthan that ofthe bacterial fluid；the chicken source lactobacillus fermentum had evident biological antagonistic action to the pig source E．coli； the pig source lactobacillusxylosusandtheCOW sourcelactobacillustrichocteshadnobiological natagonisticactionto thepigsourceE．coil． Keywords：Lactobacillus；Biologicalantagonisticaction；Oxford-cup 为了筛选性