香附对未孕大鼠离体子宫肌收缩的影响_医学论文.docVIP

香附对未孕大鼠离体子宫肌收缩的影响_医学论文 香附对未孕大鼠离体子宫肌收缩的影响_医学论文 作者：李志强，马力扬，徐敬东，杨慧霞 【摘要】 目的 探讨香附对未孕大鼠离体子宫平滑肌条运动的影响及其机制。方法 选用Wistar雌性未孕大鼠60只，体重250-300g，用Biolap410生物机能仪记录正常子宫平滑肌肌条收缩运动，并分别用苯海拉明(2×10-6mol/L)、酚妥拉明(2×10-6mol/L)、异搏定(2×10-7mol/L)、吲哚美辛(2×10-5mol/L)作用于肌条5min后，加入香附水煎醇沉液(以下简称香附)以及单用香附，观察对离体子宫平滑肌运动的影响并分析作用机制。结果 香附减弱未孕大鼠离体子宫平滑肌的收缩运动，收缩波的频率减慢，振幅减小，持续时间缩短。结论 香附减弱未孕大鼠离体子宫平滑肌的收缩运动，该作用可能是通过前列腺素的合成与释放，与L型钙通道、H1受体、α受体无关。 【关键词】 大鼠；离体子宫平滑肌；香附；苯海拉明；酚妥拉明；异搏定；吲哚美辛 　　Effect of nutgrass galingale rhizome decotion on contractile activity　of uterine smooth muscle strips of non 　　ABSTRACT: Objective To study the effects of nutgrass galingale rhizome decotion on contractile activity of uterine smooth muscle strips of nonght 250-300g to record the effects of nutgrass galingale rhizome decotion (1.0g/L, 2.0g/L, 4.0g/L, 8.0g/L) on contractile activity of uterine smooth muscle strips of nonpregnant rats with Biolap 410 Biological System. Four antagonists, diphenhydramine(2×10-6mol/L), phentolamine (2×10-6mol/L ), verapamil (2×10-7mol/L), and indomethacin (2×10-5mol/L) were used to study their mechanisms respectively. Results Water extract of nutgrass galingale rhizome (4×10-3kg/L) could significantly weaken uterine contractile activity of nonpregnant rats, lessen the frequencies, shorten the duration and lower the amplitude of contractile activity. Conclusion The effect of nutgrass galingale rhizome on contractile activity of uterine smooth muscle strips in rats may be mainly associated with prostaglandin synthese or release nothing with Lvoltagedependant calcium channels, H1 receptor or α receptor. 　　KEY WORDS: rat isolate uterine smooth muscle nutgrass galingale rhizome diphenhydramine phentolamine verapamil indomethacin 　　香附，异名雀香、莎草根等，为莎草科植物莎草(Nutgrass galingale rhizome)的干燥根茎。本品味辛、微苦、微甘，性平，具有行气解郁、调经解痛之功效。香附醇提取物对注射酵母菌及内毒素引起的大鼠发热有明显的解热作用，并有起效快、持续时间长的特点[1。此外，其挥发油对小鼠正常体温有明显的降温作用。其解热镇痛有效成分可能为三萜类化合物，香附的有效成分为α香附酮，香附流浸膏能抑制豚鼠、家兔、猫、犬等离体已孕及未孕子宫的收缩[3],但有效作用机制尚未明确。本研究将探讨其可能机制。 　　1 材料与方法 　　1.1 动物及离体子宫肌条标本准备 成