骨架蛋白对细胞表面张力和胞质分裂进程的影响.pdfVIP

骨架蛋白对细胞表面张力和胞质分裂进程的影响 1 安美文 王立 李晓娜 （山西省太原市 030024 太原理工大学应用力学与生物医学工程研究所） 摘要：为了研究胞质分裂变形中细胞极区皮层中肌动蛋白、微管和子细胞表面张力的作用，实验中向单侧子细胞极区 特定区域施加细胞松弛素D 和秋水仙素,并利用整体经过blebbistatin 处理的的细胞与对照组作比较。对不同极区皮层状态下 细胞的形态进行观察，并且分析分裂细胞的间桥变形曲线、整体变形曲线。结果显示单侧极区施加细胞松弛素D 或者秋水 仙素会抑制分裂沟内陷发生前的胞质分裂进程，在内陷发生后虽然不会抑制分裂，但是会影响细胞间桥的变形过程。破坏 单侧极区皮层的肌动蛋白装配会改变间桥变形的趋势，而极区皮层的表面张力调节着间桥变形的时间长短。破坏整体肌动 蛋白的装配会引起细胞胞质分裂的变形形式的不同。 关键词：生物力学 胞质分裂 肌球蛋白II 表面张力 Effects of Polar Cortical cytoskeleton and Imbalance Cortical Surface Tension during Cytokinesis MEIWEN AN LI WANG XIAONA LI （Institute of Applied Mechanics and Biomedical Engineering,Taiyuan University of Technology, Taiyuan, Shanxi province, China） Abstract To probe the contribution of actin and microtubule in polar cortex and the relative daughter cells surface tension to intercellular bridge thinning during cytokinesis, our study applied cytochalasin D (CD) and colchicine (COLC) in a highly localized manner to polar region of dividing normal rat kidney (NRK) cells and made use of global blebbistatin-treated cells to contrast to control. Observed the processes of cellular morphological changes and analyzed the intercellular bridge thinning trajectories of dividing cells under different polar cortical characteristic. The results showed that locally released of CD or colchicine at the polar region caused inhibition of cytokinesis before ingression. To the phases after ingression, similar treatment would allow cytokinesis to completion but dramatically influenced the trajectories of intercellular bridge thinning. Disturbing single polar cortical actin induces transformation of furrow-thinning tendency, and polar cortical tension controlled def