DNA在GO-PANI复合材料表面上的固定／杂交-.pdfVIP

第4 1 卷，第6 期 工 程 塑 料 应 用 Vol.4 1，No.6 15 2013 年6 月 ENGINEERING PLASTICS APPLICATION Jun. 2013 doi:10.3969/j.issn.1001-3539.2013.06.003 DNA 在GO–PANI 复合材料表面上的固定／杂交* 冯孝中，时宇，刘顺利，闫福丰，张治红 ( 河南省表界面重点实验室，郑州轻工业学院材料与化工学院，郑州 450002) 摘要：采用氧化还原法制备氧化石墨烯（GO ），通过原位插层聚合法制备出GO 与导电聚苯胺（PANI ）复合层状 纳米材料。采用旋涂法将GO–PANI 复合材料旋涂在自组装有十八烷三甲氧基硅烷的硅片上，对核糖核酸(DNA) 在 复合材料表面上的固定进行电化学性能测试。结果表明，DNA 能够很好地固定在GO–PANI 复合材料表面上，对将 来开发出易于制备的电化学性能优良的DNA 芯片提供了实验基础。 关键词：氧化石墨烯– 聚苯胺；纳米复合材料；DNA ；固定／杂交生物传感器 中图分类号：TB324 文献标识码：A 文章编号：1001-3539(2013)06-0015-04 DNA Immobilization Hybridization on GO–PANI Nanocomposite ／ Feng Xiaozhong ，Shi Yu ，Liu Shunli ，Yan Fufeng ，Zhang Zhihong (Henan Provincial Key Laboratory of Suface Interface Science ，Zhengzhou University of Light Industry ，Zhengzhou 450002 ，China) Abstract ：Graphene oxide(GO)was prepared by method of oxidation reduction process ，the nanocomposite of graphene oxide-polyaniline (GO–PANI) via the intercalated polymerization method in situ was constructed. In order to characterize properties of the nanocomposite easily ，GO–PANI was spin-coated onto the silicon substrate self-assembled by trimethoxyoctadecylsilane. The electrochemical properties of GO–PANI nanocomposite before and after DNA immobilization ／hybridization were detected. It suggests that probe DNA could be immobilized onto the surface of GO–PANI nanocomposite due to the electric static interaction between GO–PANI and DNA in the buffer solution. It could supply the facible method to prepare the electrochemical biosensor with good