促红细胞生成素预处理在心肌缺氧复氧损伤中的抗 - 第三军医大学学报.docVIP

促红细胞生成素预处理在心肌缺氧 复氧损伤中的抗氧化作用 秦 川 肖颖彬 陈林 钟前进 (第三军医大学附属新桥医院心血管外科，重庆400037) email:qin1976qin@, 手机电话：02368774607 [摘要] 目的：观察促红细胞生成素（erythropoietin, EPO）预处理在大鼠心肌缺氧复氧损伤（hypoxia/reoxygenation, H/R）中的抗氧化效应及其对心肌细胞凋亡的影响。方法：Wistar成年雄性大鼠60只，分为2组，分别为对照组，EPO预处理组（EPO组），每组30只，EPO组大鼠经腹腔注射5000U/kg重组人促红细胞生成素(Recombinant human erythropoietin, RHuEPO)，对照组注射同体积生理盐水。2组各15只大鼠于给药24h后，采取心肌，检测超氧化物歧化酶（superoxide dismutase, SOD）、谷胱甘肽（glutathione, GSH）以及丙二醛（malondialdehyde, MDA）含量， DNA末端转移酶标记法（TUNEL法）检测心肌细胞凋亡，免疫组化检测凋亡酶胱天蛋白酶-3（caspase-3）蛋白表达水平，每组其余15只大鼠置于常压缺氧环境中（O2 7%）12h后，移至常压常氧环境中2h，予H/R损伤，采取心肌，检测以上各指标。结果：H/R损伤前两组SOD、GSH以及MDA水平无显著差异（P0.05），心肌细胞凋亡率，心肌caspase-3蛋白表达水平在两组间亦无显著性差异（P0.05）。H/R损伤后两组心肌SOD活力及GSH含量较损伤前显著降低（P0.05，P0.01），而 MDA含量较损伤前显著升高（P0.05，P0.01），两组心肌细胞凋亡率，caspase-3蛋白表达水平也显著高于损伤前（P0.01），而EPO组的SOD活力、GSH含量高于对照组（P0.05，P0.01），MDA含量、心肌细胞凋亡率，caspase-3蛋白表达水平低于对照组（P0.05，P0.01）。结论：EPO预处理在心肌H/R损伤中具有抗氧化作用；这一作用可能是EPO预处理在心肌H/R损伤中抗凋亡保护效应的重要机制。 关键词：促红细胞生成素，心肌，缺氧复氧，超氧化物歧化酶，丙二醛 Anti-oxidation Effect of Erythropoietin Pretreatment in Myocardium Exposed to Hypoxia-reoxygenation Injury QIN Chuan, XIAO Ying-bin, Zhong Qian-jin, Chen Lin, Wang Xue-feng (Department of Cardiovascular Surgery, Xingqiao Hospital, Third Military Medical University, Chongqing 400037, China) [Abstract] Objective: This study is to explore the anti-oxidation effect of EPO pretreatment in myocardium exposed to H/R injury. The role of anti-oxidation effect in cardioprotection of EPO pretreatment was discussed, too. Method: 60 male adult Wistar rats were randomly divided into 2 groups: control group and EPO pretreatment group (EPO group). The rats in EPO group received peritoneal injection of 5000U/kg RHuEPO, and control group received the peritoneal injection of the same volume of saline. 24 hours after the pretreatment, myocardium was collected from 15 rats in each group. Apoptotic ratio of cardiomyocytes was detected by TUNEL. Expression of caspase-3 protein was analyzed by immunostaining. The contents of GSH and MDA were