化学去细胞肌肉促进大鼠脊髓半横断后的轴突再生_临床医学论文.docVIP

化学去细胞肌肉促进大鼠脊髓半横断后的轴突再生_临床医学论文 化学去细胞肌肉促进大鼠脊髓半横断后的轴突再生_临床医学论文 作者：薛辉，陈东，张秀英，刘颖 【摘要】 目的 探讨化学去细胞肌肉支架移植后对大鼠损伤脊髓轴突再生的促进作用。方法 将24只成年雄性大鼠制成脊髓半横断损伤模型后，脊髓缺损处分别植入化学去细胞肌肉、冻融去细胞肌肉和新鲜肌肉，空白组不做处理。术后4周，银染观察脊髓轴突的再生情况，对移植物中再生轴突进行计数，做统计学分析并用锇酸染色鉴定化学去细胞肌肉支架中的有髓神经纤维。另取6只化学去细胞肌肉治疗的模型鼠分别行HRP顺行和逆行示踪，1周后观察脊髓上行和下行轴突再生的情况。结果 移植术后4周，空白组无脊髓再生轴突，化学去细胞肌肉中有大量再生的轴突，而冻融去细胞肌肉和新鲜肌肉中的再生轴突很少。锇酸染色显示化学去细胞肌肉中的有髓神经纤维较少。HRP示踪证明化学去细胞支架再生的轴突来自于其上下两端的脊髓。结论 化学去细胞肌肉支架可显著促进损伤脊髓的轴突再生。 【关键词】 脊髓损伤；去细胞肌肉；移植；轴突再生 ABSTRACT: Objective To evaluate effects of chemically extracted acellular muscle graft in promoting axonal regeneration after spinal cord hemisection in adult rats. Methods Totally 24 male rats underwent spinal cord lateral hemisection. Treatment consisted of application of chemically extracted acellular muscle graft (CEAM), freezethawed acellular muscle graft (FAM), or fresh muscle graft (FM) into the lesion gap, which was left empty in lesion group (L). Rats in each group were killed 4 weeks after induction of the injury. Holmes silver staining method was performed to detect axonal regeneration. Axons in the grafts of each group were quantified, and the results were analyzed statistically. Myelinated nerve fibers in CEAM were shown by osmication staining. In another set of six rats treated with CEAM, regeneration of descending and ascending spinal fibers one week after transplantation was determined by anterograde and retrograde HRP labeling. Results In all the nonimplanted animals, no definite axonal outgrowth into the lesion was found. Many regenerating axons were noted to extend into the CEAMimplanted lesion, while the number of regenerating axons in the FAM or FM was greatly reduced. However, myelinated nerve fibers in CEAM were relatively few. HRP tracing demonstrated that the CEAM grafts could promote the regeneration of descending and ascending axons in spinal cord. Conclusion CEAM can promote robust regrowth of axons in spinal cord. KEY WORDS: spinal cord injury; acellular muscle; transplantation