匹那地尔对大鼠心肌细胞内钙及舒缩功能的影响_临床医学论文.docVIP

匹那地尔对大鼠心肌细胞内钙及舒缩功能的影响_临床医学论文 匹那地尔对大鼠心肌细胞内钙及舒缩功能的影响_临床医学论文 作者：林茹, 张泽伟, 曹春梅, 舒强, 夏强\ 【摘要】 目的探讨匹那地尔对高钾停搏液处理后大鼠离体心肌细胞内游离钙与舒缩功能的影响。方法SD大鼠心室肌细胞酶解分离静息1～2 h后随机分为对照组，高钾停搏液组，匹那地尔强化组，格列苯脲拮抗组。四组细胞均在24℃下保存2 h,此后对细胞进行复氧、复灌20 min并测定细胞收缩、［Ca2+］i瞬态、肌浆网内贮钙释放功能。结果匹那地尔使心肌细胞在缺血再灌注中收缩功能，［Ca2+］i瞬态，肌浆网内贮钙释放能力明显优于高钾停搏液组（P＜0.01〉及细胞内钙释放后的钙峰回落时间明显短于高钾停搏液组（P＜0.01〉。结论匹那地尔通过维持良好的肌浆网和细胞膜Na+/Ca2+交换体功能来调节钙瞬态变化，减少细胞内钙超载从而改善心肌细胞缺血后收缩功能，同时避免了高钾停搏液的负面效应。 【关键词】 心脏停搏液；心肌细胞；大鼠 　　Pinacidil Improves Contractile Function and Intracellular Ca2+ in Isolated Rat Cardiac Myocytes 　　Abstract: OBJECTIVEWe examined the effects of pinacidil on contractile function and intracellular calcium in isolated rat cardiomyocytes exposed to cardioplegic solution.METHODSRat myocytes were incubated at 24℃for 2 hours in cardioplegic solution with or without pinacidil (50mmol/L), then they were perfused with Krebs-Henseleit solution with a gas phase of 95% O2 / 5% CO2 at the same temperature. Parameters of contraction and intracellular calcium ［Ca2+］i transients were then measured by video tracking and spectrofluorometry. RESULTSDuring 20 minutes of perfusion after 2 hours in cardioplegic solution with pinacidil: (1) the recovery of contractile functions was significantly increased in terms of both amplitude of contraction (98.30%±9.90% vs 81.00%±11.25%, 0.05) and peak velocity of cell shortening (100.90%±13.79% vs 76.89%±18.14%, 0.01) when compared with myocytes in cardioplegic solution without pinacidil; (2) the amplitudes of the ［Ca2+］i transients evoked by electrical stimulation and caffeine (10mmol/L) increased by 23.31%～40.72% and 61.73%, respectively, over those in cardioplegic solution without pinacidil; and (3) the decay time of the caffeine-induced［Ca2+］i transient decreased by （36.64 ± 15.10）% relative to that measured in cardioplegic solution without pinadicil. The effects induced by supplementing the cardioplegic solution with pinacidil were diminished in the presence of glibenclamide (10mmol/L).CONCLUSIONAddition of