反式白藜芦醇及反式白藜芦醇苷对大鼠肝微粒体蛋白质及细胞色素P450的影响_临床医学论文.docVIP

反式白藜芦醇及反式白藜芦醇苷对大鼠肝微粒体蛋白质及细胞色素P450的影响_临床医学论文 反式白藜芦醇及反式白藜芦醇苷对大鼠肝微粒体蛋白质及细胞色素P450的影响_临床医学论文 【摘要】 目的：研究反式白藜芦醇及反式白藜芦醇苷对大鼠肝质量、肝微粒体蛋白质及细胞色素P450的影响。方法:大鼠分为空白对照组、反式白藜芦醇组及反式白藜芦醇苷组，分别连续给予等摩尔量的反式白藜芦醇、反式白藜芦醇苷(116.69、200mg/(kg·d，连续灌胃给药7d后，分光光度法测定大鼠肝质量、肝微粒体蛋白质浓度及细胞色素P450质量分数，氨基比林_N_脱甲基酶、红霉素脱甲基酶和7_乙氧基_3_异吩哐恶唑酮脱烃酶的活性。结果：反式白藜芦醇组及反式白藜芦醇苷组大鼠肝质量、肝微粒体蛋白浓度与空白对照组比无统计学意义；而2个剂量组大鼠肝微粒体中细胞色素P450、氨基比林_N_脱甲基酶、红霉素_N_脱甲基酶和7_乙氧基_3_异吩哐恶唑酮脱烃酶的活性与空白对照组比有统计学意义(0.05，对细胞色素P450酶有抑制作用，但剂量组间无统计学意义。结论：反式白藜芦醇与反式白藜芦醇苷可引起肝药酶对某些药物代谢的改变。 【关键词】 反式白藜芦醇；反式白藜芦醇苷；肝质量；肝微粒体蛋白质；细胞色素P450 ［Abstract］Objective：To study the effects of trans_resveratrol and trans_piceid on liver weight, contents of microsomal protein and cytochrome P450. Methods: Rats were divided into trans_resveratrol, trans_piceid and control groups. The UV method was used to detect the liver weight, contents of microsomal protein and cytochrome P450, the activities of aminopyrine_N_demethylase, erythromycin_N_demethylase and coumarin 7_hydroxylation of rats, after administration with equimolar trans_resveratrol(116.69 mg/(kg·dand trans_piceid(200 mg/(kg·dfor 7 days. Results: The study showed no significant differences on liver weight and contents of microsomal protein among three groups. Compared with control group, the activities of cytochrome P450,aminopyrine_N_demethylase, erythromycin_N_demethylase and coumarin 7_hydroxylation between two dosage groups(0.05had statistical significance. But there was no significant difference between two dosage groups. Conclusion: Trans_resveratrol and trans_piceid can result in alteration of metabolism of some drugs affected by drug metabolizing enzyme of liver. ［Key Words］trans_resveratrol，trans_piceid，liver weight，microsomal protein，cytochrome P450体内、外实验表明，白藜芦醇及白藜芦醇苷具有广泛的生物学活性，因而近年来对它们相应的稳定单体反式白藜芦醇及反式白藜芦醇苷的药理作用研究不断深入，主要涉及抗氧化、抗炎、抗癌、心血管保护及神经保护等多方面［1_2］，但对其在生物体内药代动力学过程还知之甚少。肝药酶分Ⅰ相和Ⅱ相药物代谢酶，细胞色素P450(CYP是Ⅰ相中催化多种药物、前毒物、前致癌物等外源性物质的主要酶类，主要有CYP1A2、CYP2A6、CYP2B6、CYP2C、CYP2D6、CYP2E1、CYP3A等，本文考察大鼠连续应用反式白藜芦醇及反式白藜芦醇苷后，对其肝质量、肝微粒体蛋