反相高效液相色谱法同时测定脉络宁注射液中6种酚酸类成分的含量_临床医学论文.docVIP

反相高效液相色谱法同时测定脉络宁注射液中6种酚酸类成分的含量_临床医学论文 反相高效液相色谱法同时测定脉络宁注射液中6种酚酸类成分的含量_临床医学论文 作者：张蕙,李祥,周红燕,徐向阳,陈伟奇 【摘要】 目的 建立用反相高效液相色谱法同时测定脉络宁注射液中6种酚酸含量的方法。方法 采用Kromasil-C18柱(4.6 mm×250 mm,5 μm)；流动相：乙腈-0.1%磷酸溶液,梯度洗脱；流速：1.0 mL/min；紫外检测波长：327 nm；柱温：25 ℃；进样量：20 μL。结果 新绿原酸、绿原酸、隐绿原酸、咖啡酸、3,5-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸的线性范围分别为0.010 90～0.544 8 μg(r＝0.999 9)、0.024 06～1.203 2 μg(r＝1)、0.011 06～0.552 8 μg(r＝1)、0.015 94～0.796 8 μg(r＝1)、0.009 104～0.455 2 μg(r＝1)、0.010 37～0.518 4 μg(r＝1),回收率分别为101.06%、100.22%、101.54%、99.42%、100.21%、101.65%。结论 本法灵敏简便,准确可靠,可作为脉络宁注射液中新绿原酸、绿原酸、隐绿原酸、咖啡酸、3,5-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸含量同时测定的方法 【关键词】 脉络宁注射液；新绿原酸；绿原酸；隐绿原酸；咖啡酸；3,5-二咖啡酰奎宁酸；3,4-二咖啡酰奎宁酸；反相高效液相色谱法 Abstract：Objective To establish a method for determination the contents of six phendic acids in Mailuoning injection by HPLC at same time. Methods Separation was performed on a Kromasil-C18 (4.6 mm×250 mm, 5 μm) column with mobile phase consisting of 0.1% phosphoric acid-water and Acetonitrile with gradient elute at the flow rate of 1 mL/min. The UV detection wavelength was set at 327 nm and the column temperature was set at 25 ℃. The sample size was 20 μL. Results The standard cures of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, 3,5-Dicaffeoylquinic acid, 3,4- Dicaffeoylquinic acid were linear within the contentration range of 0.010 90～0.544 8 μg (r＝0.999 9), 0.024 06～1.203 2 μg (r＝1), 0.011 06～0.552 8 μg (r＝1), 0.015 94～0.796 8 μg (r＝1), 0.009 104～0.455 2 μg (r＝1), 0.010 37～0.518 4 μg (r＝1) respectively. And the recovery rates of them were 101.06%, 100.22%, 101.54%, 99.42%, 100.21%, 101.65% respectively. Conclusion The method appeared to be simple, reliable, accurate and can be used to determine the contents of six phendic acids in Mailuoning injection. Key words：Mailuoning injection；neochlorogenic acid；chlorogenic acid；cryptochlorogenic acid；caffeic acid；3,5-Dicaffeoylquinic acid；3,4- Dicaffeoylquinic acid；RP-HPLC 脉络宁注射液主要由玄参、金银花、牛膝、石斛组成,具有清热养阴、活血化瘀的功效,用于治疗血栓闭塞性脉管炎、静脉血栓、动脉硬化性闭塞症、脑血栓及后遗症等。方中主药金