囊性纤维化跨膜转导调节因子表达率与人精子受精能力的关系_临床医学论文.docVIP

囊性纤维化跨膜转导调节因子表达率与人精子受精能力的关系_临床医学论文 囊性纤维化跨膜转导调节因子表达率与人精子受精能力的关系_临床医学论文 作者：李楚艳，李坤，陈璋辉，于建民，吕建新，倪崖 【摘要】 目的?：研究人精子囊性纤维化跨膜转导调节因子(cystic fibrosis transmembrane conduc-tance regulator，CFTR)表达率与精子受精能力间的关系。方法：运用间接免疫荧光法及蛋白印迹分析法研究CFTR在人精子上的表达及其在不同类型(生育组、不明原因性不育组及不育组)精子上的表达率情况。结果：通过间接免疫荧光法发现CFTR表达在人精子赤道板上，蛋白印迹分析进一步证实人精子中存在CFTR，分子量为170 kD;采用间接免疫荧光技术，CFTR在生育组、不明原因性不育组及不育组(包括畸形精子症、弱精症、少精症)上的表达率有显著差异：CFTR在生育组精子上的表达率(88.13%±3.79%)远高于不明原因性不育组(52.93%±15.36%)及不育组(35.89%±20.41%)(P?lt;0.01)。结论：CFTR表达率下降与人精子受精能力降低有关。 【关键词】 囊性纤维化跨膜转导调节因子;表达率;人精子;受精能力 　Abstract: ? Objective: To investigate the correlation between percentage of human sperm expressing CFTR and sperm fertilizing capacity. Methods: The expression of CFTR on spermatozoa was conducted by indirect immunofluorescence staining and Western Blot Analysis. And the percentage of spermatozoa expressing CFTR from fertile， unexplained infertile and infertile men?(mainly teratospermic， asthenospermic and oligospermic)?was examined with indirect immunofluorescence staining. Results:CFTR protein was localized to the equatorial segment of sperm head and molecular weight was approximately 170 kD. And the percentage of spermatozoa expressing CFTR in fertile men was signifi-cantly different from that in the unexplained infertile men and infertile men categories，the fertile men spermatozoa greatly higher?(88.13%±3.79%)than that the unexplained infertile men (52.93%±15.36%) and infertile men (35.89%±20.41%) (P?lt;0.01). Conclusion: The defective expression percentage of CFTR in human sperm is related to a reduction of sperm fertilizing capacity. 　　Key words: ? CFTR;expression percentage;human sperm;sperm fertilizing capacity 　　囊性纤维化病(cystic fibrosis，CF)是由囊性纤维化跨膜转导调节因子(cystic fibrosis transmembrane conduc-tance regulator，CFTR)基因突变引起的一种遗传性疾病，其临床表现是进行性肺衰竭、胰腺功能不全及两性不育等。将近97%男性CF患者因为先天性双侧输精管缺失(CBAVD)[1-2]、先天性单侧输精管缺失(CUAVD)[3]及杨氏综合征(Young’s syndrome)[4]而致不育。Van der Ven等[5]报道17.5%男性不育是因为精子质量下降，14.3%少精症患者至少有一个CFTR基因突变，这些结果指出CFTR除了对附睾腺及输精管发生有重要作用以外，也可能参与精子的