四君子汤对烟曲霉菌体外生长的抑制作用_临床医学论文.docVIP

四君子汤对烟曲霉菌体外生长的抑制作用_临床医学论文 四君子汤对烟曲霉菌体外生长的抑制作用_临床医学论文 作者：余克花 王鹏刘金辉 谢小梅，汪雁 【摘要】 目的探讨中药方剂四君子汤是否可以抑制体外培养的烟曲霉菌的生长。方法一定剂量的四君子汤和氟康唑处理的103个烟曲霉菌孢子涂布在沙氏固体培养基培养4 d，计算并比较烟曲霉菌生长形成的菌落数量、菌落大小、镜下计数孢子柄与孢子的数量。结果与对照组相比，四君子汤和氟康唑可以显著降低烟曲霉菌的菌落数量和菌落大小、抑制孢子柄与孢子的的形成(0.05)，结果呈现剂量-效应关系。与氟康唑处理组相比， 300 μl四君子汤处理的分生孢子形成的菌落数、菌落大小、孢子柄和产生的分生孢子数均显著低于氟康唑处理组( 0.05)。结论中药方剂四君子汤可以明显地抑制烟曲霉菌的体外生长，提示四君子汤在治疗烟曲霉菌所致的肺部感染具有潜在的应用价值。 【关键词】 四君子汤;烟曲霉菌;分生孢子 　Abstract：ObjectiveTo explore the inhibitory effect of Si-jun-zi decoction on growth of Aspergillus fujmigatus in vitro.Methods103 conidia of Aspergillus fumigatus treated with different concentrations of Si-jun-zi decoction and Fluconazole were plated on the Sabouraud dextrose agar and cultivated for 4 d.The number and size of colony were measured. The number of conidia and conidiophore were counted under microscope.ResultsCompared with control group,Si-jun-zi decoction and fluconazole could decrease colony number and size significantly and inhibit the formation of conidia and conidiophore(0.05).The inhibition effect was dose-dependent.The number and size of colony,the number of conidia and conidiophore in the group treated with 300μl Si-jun-zi decoction were significantly lower than those in the group treated with fluconzaole(0.05).ConclusionSi-jun-zi decoction can inhibit significantly in vitro growth of Aspergillus fumigatus ,suggesting that Si-jun-zi decoction has potential therapeutic value against lung infection by aspergillus fumigatus. 　　Key words：Si-jun-zi decoction;Aspergillus fumigatus;Conidium 　　烟曲霉菌是导致免疫低下患者肺部感染，侵袭性肺曲霉病(Invasive Pulmonary Aspergillosis, IPA)的常见真菌。IPA死亡率高达80%～90%，预后很差[1]。四君子汤作为传统的中药补益方剂，能明显改善机体免疫功能[2]。动物实验研究发现四君子汤通过提高机体免疫功能，治疗烟曲霉菌感染导致的IPA 具有一定的效果[3]。四君子汤对烟曲霉菌生长的直接抑制作用作为治疗IPA的一种机制还未见报道。本研究将一定剂量的四君子汤处理的烟曲霉菌孢子涂布在沙氏固体培养基进行培养，通过计算烟曲霉菌生长形成的菌落数量及测量菌落大小、镜下计数分生孢子柄与分生孢子的数量，以分析四君子汤是否可以抑制烟曲霉菌的体外生长，为将中药补益方剂用于治疗难治性的IPA病积累实验数据。 　　1材料 　　1.1沙氏固体培养基16 g葡萄糖，4.0蛋白胨，7.2 g琼脂，加蒸馏水至400 ml，高压灭菌后，无菌条件下倒平板，制成沙氏固体培养基4℃冰箱保存。 　　1.2菌株烟曲霉菌临床分离株，分离号：3910，购自中国医学真菌保藏中心