基质金属蛋白酶14和细胞外基质金属蛋白酶诱导因子在视网膜母细胞瘤中的表达及意义_临床医学论文.docVIP

基质金属蛋白酶和细胞外基质金属蛋白酶诱导因子在视网膜母细胞瘤中的表达及意义_临床医学论文 基质金属蛋白酶和细胞外基质金属蛋白酶诱导因子在视网膜母细胞瘤中的表达及意义_临床医学论文 【摘要】 目的 探讨基质金属蛋白酶（matrix metalloproteinase）和细胞外基质金属蛋白酶诱导因子（extracellular matrix metalloproteinase inducer,EMMPRIN）在视网膜母细胞瘤（retinoblastoma, Rb）中的表达及其与临床病程的关系。方法 用免疫组织化学SP法分别检测39例Rb中MMP及EMMPRIN的表达；用LEICA IM 50型全自动医学图像分析系统测量其平均灰度值，比较不同临床和病理阶段Rb中表达MMP和EMMPRIN的差异及二者阳性表达之间的关系。结果 39例Rb中MMP阳性表达34例，占87.18%；EMMPRIN阳性表达33例，占84.62%；青光眼期Rb中MMP和EMMPRIN的表达明显高于眼内期（P＜0.01及P＜0.05），眼外期二者表达明显高于青光眼期（P＜0.01及P＜0.05）；视神经浸润组Rb中MMP和EMMPRIN的表达明显高于无视神经浸润组（P＜0.01及P＜0.05）；Rb中MMP和EMMPRIN的阳性表达水平呈密切相关（P＜0.01）。结论 MMP及EMMPRIN的表达水平可能与Rb的浸润转移有关，二者共同促进Rb的浸润转移。 【关键词】 视网膜母细胞瘤；基质金属蛋白酶；细胞外基质金属蛋白酶诱导因子；免疫组织化学　 　　To investigate the expressions of matrix metalloproteinaseoteinase inducer (EMMPRIN) in retinoblastoma(Rb) and the relationships between MMP14 and EMMPRIN and tumor development. Methods Immunohistochemical technique was used to determine the expressions of MMP14 and EMMPRIN in 39 cases of paraffin embedded Rb samples. Quantitative analysis of the expressions of MMP14 and EMMPRIN were assessed by measuring the mean gray scale of Rb tissue with the LEICA IM 50 Color Pathologic Analysis System. Differences of the expressions of MMP14 and EMMPRIN in each clinical and pathological stage were statistically analyzed, and the same step was also undertaken to study the relationship between Rb with MMP14 positive expression and that with EMMPRIN positive expression. Results Positive expression rate of MMP14 was 87.18% (34/39) and of EMMPRIN was 84.62%(33/39). The expressions of MMP14 and EMMPRIN were significantly higher in tumors of the glaucomatous stage than those in the intraocular stage (P＜0.01 and P＜0.05), and the same conclusion can be concluded between those in the extra＜0.01 and P＜0.05), also the expressions of MMP＜0.01 and P＜0.05). There was a statistically significant positive correlation between the 　　视网膜母细胞瘤（retinoblastoma,Rb）起源于视网膜胚胎性核层细胞，是一种婴幼儿最常见的、危害性最大的眼内恶性肿瘤，可危及患儿的视力甚至生命。通常认为，Rb患儿的预后与视神经浸润与否、有无眶内及