多糖生物膜在骨组织工程中应用的实验研究_临床医学论文.docVIP

多糖生物膜在骨组织工程中应用的实验研究_临床医学论文 多糖生物膜在骨组织工程中应用的实验研究_临床医学论文 作者：倪建光，刘丹平，韩宝芹 【摘要】 　　目的 在骨组织工程的载体外包裹多糖生物膜(PSBM)，减少种子细胞在复合载体时的外溢逃出，验证其在组织工程中应用的可行性。方法 用全骨髓法获得骨髓基质干细胞，体外扩增后，取第三代细胞种植于支架材料载体多孔纳米磷灰石－硅灰石生物活性玻璃陶瓷/壳聚糖复合支架材料（A-W-MGC/CS）上，分2 组，实验组载体外用多糖生物膜物理包裹，对照组不包裹多糖生物膜，观察载体外培养皿上的细胞生长数量；MTT检测细胞活力；电镜观察载体上细胞粘附生长情况。结果 实验组培养板上载体外漏细胞较少，对照外漏细胞较多：MTT检测两组细胞活力差别无显著性（P＞0.05）；电镜观察实验组载体上细胞粘附生长较对照组稍优。结论 多糖生物膜在骨组织工程中具有应用前景。 【关键词】 多糖生物膜 骨髓基质干细胞（BMSCs） 支架材料 组织工程 　　Abstract: Objective To pack bone tissue engineering carrier with polysaccharide biomembrane to decrease seed-cells leaking, and to authenticate the feasibility of applying polysaccharide biomembrane in bone tissue engineering. Methods Bone marrow stromal cells were derived through whole-bone-marrow method, and, after amplification in vitro, the cells of the third generation were implanted in A-W-MGC/CS. Then the cells were divided into two groups-an experimental group with carrier packed with PSBM and a control group with carrier un-packed. The number of cell growing in culture dish out of the carrier was observed，the state of cell vigor was detected with MTT, and the state of cell adhesion was observed with electron microscope. Results The number of leaking cell in the experimental group was less than that in the control group. The result of MTT detection showed that difference of cell vigor between two groups was un-significant (P＜0.05). The growing state of cell in the experimental group was better than that in the control group under electron microscope. Conclusions Polysaccharide biomembrane has an applied prospect in Bone tissue engineering. 　　Key words：polysaccharide biomembrane; bone marrow stromal stem cell; bracket material; tissue engineering 　　骨组织工程的核心是在体外培养细胞，然后将细胞种植到具有一定结构的三维支架上，再将这种细胞生物材料复合体植入骨缺损部位，通过生物过程使细胞长入多孔支架中，即经过细胞间相互粘附、增殖、分化，分泌细胞外基质最终形成具有一定结构和功能的组织或器官，从而达到骨修复的目的。细胞和支架材料的相互作用又起到关键的作用，减少细胞复合材料又未贴附在支架材料这段时间的外漏，可以有效的减少种子细胞的损失，增加细胞与支架材料之间的作用时间，更利于细胞粘附。由中国海洋大学生命学院研制的多糖生物膜（polysaccharide biomembrane,PSBM）是一种安全无毒，具有很好生物相容