大鼠外伤性白内障晶状体上皮细胞中MMP2和TIMP2的表达_临床医学论文.docVIP

大鼠外伤性白内障晶状体上皮细胞中MMP和TIMP的表达_临床医学论文 大鼠外伤性白内障晶状体上皮细胞中MMP和TIMP的表达_临床医学论文 作者：石云峰，王峰，谢志，张爽，廖伟 【摘要】 　　目的：探讨基质金属蛋白酶（matrix metalloproteinase，MMP）及其抑制剂（tissue inhibitor of metalloproteinase，TIMP）在大鼠外伤性白内障形成过程中所发挥的作用。方法：正常成年SD大鼠20只，体质量250～300g，建立单眼晶状体穿通伤后外伤性白内障模型，并随机分为伤后12，24，72，168h 4个时相组。用免疫组织化学染色法检测MMP和TIMP在各组晶状体上皮细胞（lens epithelial cell, LEC）中的表达情况。结果：外伤后12h晶状体开始出现混浊，并随时间延长而加重。MMP在各外伤组LEC中均有较强的阳性表达，而在正常对照眼中无表达。TIMP的表达在各组LEC中差异均无显著意义（Pgt;0.05）。MMP与TIMP阳性表达率间无相关性（Pgt;0.05）。 结论：MMP参与了外伤性白内障形成的病理环节。MMP和TIMP表达失衡在外伤性白内障的发生和发展过程中发挥重要作用。 【关键词】 基质金属蛋白酶；基质金属蛋白酶抑制剂；外伤性白内障；晶状体上皮细胞 　　AbstractMETHODS:Twenty healthy SD rats(weighing 250300g) were used to establish traumatic cataract model on left eyes and then divided into posttraumatic 12, 24, 72 and 168 hours groups randomly. The expression of MMP2 and TIMP2 in lens epithelial cells(LEC) were examined by immunohistochemistry staining.RESULTS: In the posttraumatic 12 hours, lens opacity began to appear and aggravated as the time went by.Immunohistochemical results showed positive MMP2 expression in the LEC of every traumatic groups. No expression of MMP2 was detected in the compared eyes without surgery. But there was no statistical significance for TIMP2 expression (Pgt;0.05). There was no correlation between the expression of MMP2 and TIMP2 (Pgt;0.05). CONCLUSION: MMP2 takes part in the formation process of traumatic cataract. The imbalance between the expression of MMP2 and TIMP2 may play a critical role in the generation and development of traumatic cataract.  　　KEYWORDS: matrix metalloproteinaseoproteinase2; traumatic cataract; lens epithelial cell 　　0　引言 　　基质金属蛋白酶（matrix metalloproteinase）是降解细胞外基质（extracellular matrix, ECM）和基底膜的一类重要酶类，金属蛋白酶组织抑制剂（tissue inhibitor of metalloproteinase）是MMP的特异性抑制物，ECM的稳定主要依赖于MMP/TIMP间的动态平衡。MMP/TIMP系统在多种眼科疾病中所起的作用是近年来的研究热点。外伤性白内障是眼球发生穿孔伤或钝挫伤后常见并发症之一，发病率为36%～52%，也是眼外伤致盲的主要原因之一，占视力致残者的28.1%。许多研究表明机械性损伤引起的晶状体上皮细胞（lens epithelial cell，LEC）增生、移行和化生与外伤性白内障的发生发展密切相关，但目前尚无MMP和TIMP在外伤性白内