天麻钩藤饮对帕金森病大鼠神经行为学及氧化应激反应的影响_临床医学论文.docVIP

天麻钩藤饮对帕金森病大鼠神经行为学及氧化应激反应的影响_临床医学论文 天麻钩藤饮对帕金森病大鼠神经行为学及氧化应激反应的影响_临床医学论文 作者：王文武 何建成 丁宏娟 【摘要】 　　目的 观察天麻钩藤饮对帕金森病(PD)大鼠神经行为学及氧化应激的影响。方法 采用6羟基多巴胺(6注射于脑右侧黑质造成单侧PD损毁模型，并用中药天麻钩藤饮（浓度为5.13 g/ml）进行治疗，共给药14 d。同时设立正常对照组、假手术组，观察各组大鼠神经行为学的变化，同时运用化学比色法测定大鼠中脑黑质、纹状体部位活性氧、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH）、超氧化物歧化酶（SOD）、丙二醛（MDA）的活性。结果 神经行为学方面，模型组大鼠治疗前后旋转圈数无显著差异；天麻钩藤饮组大鼠旋转圈数较模型组显著减少（P＜0.05），且治疗前后比较差异显著（P＜0.05）；模型组活性氧、MDA明显升高，GSH、GSH、SOD明显降低，与正常对照组、假手术组比较，差异显著（P＜0.05或P＜0.01）；而天麻钩藤饮对其均有明显的改善作用（P＜0.05）。结论 天麻钩藤饮可以明显改善PD大鼠的神经行为学变化，并可以提高机体的抗氧化和清除自由基的能力。 【关键词】 天麻钩藤饮；帕金森病；神经行为学；氧化应激；大鼠 【Abstract】 Objective To observe effect of tianma gouteng drink on the behavioural and oxidation stress response of Parkinson’s disease (PD) rat.Methods Rats were divided into normal control, sham operation, model, and treatment groups (6 rats in each group). PD rats were produced by injection of 6OHDA stereotaxically into the right side of the mesencephic ventral tegmental area (VTA ) and substantia nigra pars compacta (SNc) and treated with tianma gouteng drink (5.13 g/ml) for 14 days. Behavioural changes and the activity of reactive oxygen species (ROS), glutathione (GSH), glutathione peroxidase (GSHPx), superoxidase dismutase (SOD), malonaldehyde (MDA) of the substantia nigra and corpus striatum of the PD rats were observed. Results There was no significant difference in rotation behaviour between before and after treatment in model group (P＞0.05). Compared to model group, the rotation behaviour was significantly decreased in treatment group, and there was significant difference between before and after treatment in treatment group (P＜0.05). Compared with the normal control and sham operation groups, ROS and MDA contents were obviously increased and GSH, GSH＜0.05). However, the above indicators were improved in treatment group (P＜0.05). Conclusions Tianma gouteng drink can significantly improve the neurological behavior, and enhance anti 【Key words】 Tianma gouteng drink；Parkinson’s disease；Neurological behavior； Oxid