枯草芽孢杆菌S44抗菌粗蛋白地研究.pdfVIP

枯草芽孢杆菌S44抗菌粗蛋白的研究 罗燕娜1任娟2 孙佰平2 赵思峰2 1农六师农科所，新疆五家渠831300 2石河子大学绿洲农作物病害防控重点实验室，新疆石河子832003 摘要 为了明确实验室前期筛选获得的枯草芽孢杆菌S44对棉花主要土传病害的防病机理，对其活性功能蛋白进行了分离、 纯化和生理生化特性的测试，结果表明，其抗菌粗蛋白经120℃处理30min后仍能保持较高的抑菌活性；对蛋白酶K不敏感； 紫外照射和可见光均不影响其抑菌活性；经甲醇、乙醇等多种有机溶剂处理后活性不丧失；同时该抗菌粗蛋白能有效抑制立 枯丝核菌、棉花枯萎病菌、西瓜枯萎病菌、棉花黄萎病菌、番茄早疫病菌、向日葵菌核病菌6种病菌菌丝的生长。 关键词 枯草芽孢杆菌；抗菌粗蛋白；棉苗立枯病；棉花黄萎病 S432.1;S-3 A ResearchontheAntagonisticFunctionalCrudeProteinof BacillusSubtilisS44 LuoYanna1 RenJuan2 SunBaiping2 ZhaoSifeng2 1AgriculturalScientificResearchInstituteoftheSixDevision,WuJiaqu,Xinjiang831300 2TheKeyLaboratoryofPreventionandControlforOasisCrop Disease,ShiheziUniversity,Shihezi,Xinjiang832003 Abstract TomakeclearthepreventionmeehanismofbacillussubtilisS44tomajorsoilbornediseaseofcotton,separation,purifica tion,physiologicalandbiochemicalcharacteristicsofS44antagonisticfunctionalcrudeproteinwerestudied.TheresultsshowthatS44 crudeproteinwasthermostableforithadstronginhibitoryactivitiesaftertreatedat120℃ for30min.ProteinaseKhadnoeffectonits activity.Theinhibitorytiterofproteinexposeduderultravioletradiationwassimilartovisiablelight.Theantagonisticeffectofprotein treatedwithvariouskindsoforganicsolventwasdisplayedthatitwasstableinmethanol,ethnol,acetone,ethylacetateandsoon.Sev eralpathogenswerestronglyinhibitedbytheprotein,suchassclerotiniasclerotiorum,RhizoctoniasolaniKuhn,fusariumoxysporum f.sp.vasinfectum,fusariumoxysporumf.sp.niverum,verticilliumdahliaekelb,alternariasolanietc. Keywords Bacillussubtillus;antagonisticfunctionalcrudeprotein;Rhizoctoniasolani-induceddamping-offofcotton;cottonVer ticilliumwilt 第2属 罗燕娜等；桔草芽孢杆菌s“抗菌粗蛋白的研究 171 不仅造成烂种、烂芽和棉苗死亡，严重时导致大面积 肉膏5g、氯化钠5g、甘氨酸0．1g，蒸馏水 棉田