茎瘤芥(榨菜)游离小孢子培养胚胎发生能力地研究.pdfVIP

茎瘤芥 （榨菜）游离小孢子培养胚胎发生能 力的研究 沈进娟 范永红 冉广葵 林合清 刘义华 王旭祎 周光凡 重庆市涪陵区农业科学研究所；南方芥菜品种改良与栽培技术国家地方联合工程实验室；榨菜品种改良栽 培技术重庆市工程实验室，重庆涪陵408000 摘要：试验对9个基因型的茎瘤芥材料进行游离小孢子培养，探讨基因型、蔗糖浓度、甘露 醇、活性炭、花蕾大小及培养基灭菌方式等因素对茎瘤芥小孢子胚胎发生能力的影响。实验结果表 明：初花期至盛花期小孢子成胚率最高；单独提高提取液中蔗糖浓度或者在提取液中添加8％甘露 醇都能增加小孢子膨大数目，同时使用可以大幅度提高小孢子膨大数目；添加活性炭的浓度在 0.02g/L左右时最佳，过高和过低都不利于小孢子发育；过滤灭菌可以较好的保持培养基的成分不 变，显著优于高压灭菌；供体植株基因型是影响小孢子胚状体产生的关键因素，不同基因型之间差 异显著，早熟品种容易被诱导胚胎发生，越晚熟品种胚胎发生难度愈大。 关键词：茎瘤芥；游离小孢子；胚胎发生；胚状体 StudyonMicrosporeEmbryogenicAbilityinIsolatedMicrospore CultureofTuberMustard SHENJin-juanFANYong-hongRANGuang-kuiLINHe-qingLIUYi-huaWANGXu-yi ZHOUGuang-fan FulingAgriculturalScienceInstituteofChongqing,SouthMustardVarietiesImprovementand CultivationTechniquesofNationalLocalUnitedEngineeringLaboratory,TuberMustard VarietiesImprovementandCultivationTechniquesofChongqingEngineeringLaboratory,Fuling, Chongqing,408000,China Abstract:Isolatedmicrosporesof9genotypesoftubermustardwereusedfortissuecultureto studytheeffectsofgenotype,sucroseconcentration,mannitol,activatedcarbon,budsize,medium sterilizationmethodonembryogenesis.Theresultsshowedthatthefloweringstagetoblooming microsporeembryowasthehighest;aloneimprovesextractconcentrationofsucroseorextract containing8％ mannitolcouldincreasethenumberofmicrosporeenlargement,simultaneoususe couldgreatlyimprovethenumberofmicrosporeenlargement;addingactivecarbonconcentrationof about0.02g/Lwasthebest,toohighandtoolowweredetrimentaltothedevelopmentofmicrospore; filteringsterilizationcouldkeepbetterculturemediumcompositionunchanged,significantlybetter thanthehighpressuresterilization;effectsofdonorplantgenotypewerethekeyfactorsof microsporeembryoidgeneration,therewassignificantdifferencebetweendifferentgenotypes,early maturingvarietywaseasilyinducedembryogenesis,andthelatematuringvarietieswasmore difficult. Keywords:tubermustard;isolatedmicrospore;embryogenesis;embryoid 中期至双核早期的在 ％ (w/v)饱和次氯酸韦 j-，J、抱于堵乔，与小刀口 孽养过