HPLC法测定天元克痛方主要成分地研究.pdfVIP

HPLC 法测定天元克痛方主要成分的研究 方翼 1 王睿 徐伟 杨明会 张海燕 解放军总医院临床药理药学研究室 北京 100853 摘要 目的 建立天元克痛方的主要成分的含量测定方法 方法 应用 HPLC 法测定对方中的主要成分延胡索乙素和东莨菪碱进行含量测定 考察方法线形范 围 精密度及回收率等 结果 采用 HPLC 法对延胡索乙素进行测定 标准曲线 为 y=0.0774x+1.1044;r=0.9999 高 中 低三个浓度的日间精密度分别为 0.15% 0.3% 0.4% 日内精密度分别为 0.43% 0.69% 0.58% 回收率分别为 100.8% 101.2% 104.5% HPLC 法对东莨菪碱进行含量测定 标准曲线为 y=0.0667x-0.6352;r=0.9999 高 中 低三个浓度日内精密度分别为 3.04% 1.66% 0.46% 回收率分别为 104.34% 95.05% 95.79% 结论 采用 HPLC 法对天 元克痛方中主要成分延胡索乙素和东莨菪碱进行含量测定 方法灵敏可靠 重现 性好 关键词 天元克痛方 HPLC 延胡索乙素 东莨菪碱 Determination of Content of the Tian Yuan Ke Tong Fang by HPLC Fang Yi Wang Rui Xu Wei Yang Ming-hui Zhang Hai-yan (Department of Clinical Pharmacology, Chinese PLA General Hospital,Beijing 100853) Abstract Objective: To establish the measurement method of Tian Yuan Ke Tong Fang, which is a new drug of the traditional Chinese medicine. Method: HPLC was used to determine corydalis B and scopolamine which are the main components of the Tian Yuan Ke Tong Fang. Results: HPLC was used to determine the corydalis B, the equation of standard curve is y=0.0774x+1.1044 (r=0.9999). The intraday precisions of different concentration are 0.15%, 0.3%, 0.4%; The interday precisions of different concentration are 0.43%, 0.69%, 0.58%; The recovery rates are 100.8%, 101.2%, 104.5%. HPLC was used to determine the scopolamine, the equation of standard curve is y=0.0667x -0.6352 (r=0.9999). The intraday precisions of different concentration are 3.04%,1.66%,0.46%; The interday precisions of different concentration are 0.84%,0.96%,1.43%; The recovery are 104.34%,95.05%,95.79%. Conclusion: HPLC was used to determine the corydalis