中药生姜活性成份姜酚对胶原诱导性关节炎抗炎作用的研究.pdfVIP

中药生姜活性成份姜酚对胶原诱导性关节炎抗炎作用研究 贵阳中医学院第二附属医院风湿免疫科 （贵阳550003） 姚血明 马武开 唐芳 周静 刘丽敏 龙小琴 黄颖 陆道敏 摘要 目的：探讨姜酚对牛Ⅱ型胶原诱导性关节炎大鼠(CIA)的作用及机制。方法：建立CIA 模型大鼠，观察姜酚对CIA大鼠关节病理、关节炎指数变化，ELISA法检测CIA大鼠血清TNF-α、IL-1 及IL-1β的含量。结果：姜酚能够抑制CIA大鼠足趾肿胀，降低关节炎指数，并能降低血清TNF-α、 IL-1及IL-1β水平，明显减轻关节滑膜细胞增生、滑膜层及滑膜下层淋巴细胞浸润，与雷公藤多苷对 照组相当(P＞0.05)。结论：姜酚对CIA大鼠关节炎具有治疗作用，其疗效与雷公藤多苷对照组相当， 具有一定临床应用前景。 关键词：姜酚 ; 胶原诱导性关节炎；TNF-α ; IL-1 ; IL-1β StudyonAnti-inflammatoryEffectofGingerolCapsuletoCollagen-inducedArthritis MAWu-kaiYAOXue-mingTANGFangZHOUJingLIULi-mingLongXiao-qing HUANGYingLU Dao-ming TheSecondAffiliatedHospitalofGuiYangcollegeofTCM,Guiyang550001China Abstract Objective:ResearchontheinfluenceofGingerphenolcapsuletothearthritis index,jointswellingandexpressionofTNFalpha、 IL-1andIL-1β incollageninducedarthritis rats.Method:40femalesWistarratsdividedrandomlyinto4groups,blankgroup、 modelcontrol group、 gingerphenolcapsuletreatmentgroupandtripterygiumglucosidescontrolgroup.CIA modelswasEstablishedInordertoobservethegingerolCapsuleonCIAratanklepathology, arthritisindexandserumTNF-α,IL-1,IL-1βlevelsinrats..Results:Gingerolcapsules caninhibitCIAratstoeswelling,reducedarthritisindex,andcanreducetheserumlevels ofTNF-o,IL-landIL-1β levels,significantlyreducethesynovialcellsproliferation, synoviallayerandsynoviallowerlymphocyteinfiltration,andtripterygiumglycosidesgroup A(P＞0.05).Conclusion:ThetreatmentofGingerphenolcapsuletoCIAratshasremarkable curativeeffect.Theremaybearelationshipbetweeneffectandthecontrolexpressionof CIAratsserumTNF-a、 IL-1andIL-1β inflammationfactors. Keywords:Gingerphenolcapsule ;Collageninducedarthritis ; TNF-a;IL-1;IL-1β