10 个小麦新品种(系) 抗小麦叶锈性评价.pdfVIP

中国农业科学 2011,44(14):2900-2908 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.14.006 10 个小麦新品种 （系）抗小麦叶锈性评价 师丽红，张 娜，胡亚亚，杨文香，刘大群 (河北农业大学植物保护学院植物病理系分子植物病理学实验室/河北省农作物病虫害生物防治工程技术研究中心，河北保定 071001) 摘要：【目的】明确小麦新品种 （系）河农 5290、河农58-3、河农825、河农826、河农827、河农6049、 河农6251、河农6425、河农7106 和河农9206 的抗叶锈性，确定其应用潜力，为合理推广使用这些品种 （系）提 供依据。【方法】采用16 个具有鉴别能力的小麦叶锈菌株对测试的10 个材料进行苗期抗性鉴定和基因推导；选用 其中6 个菌株对其进行田间成株期抗叶锈性鉴定和基因推导，同时，结合使用已报道的15 个抗叶锈病基因稳定的 分子标记对测试材料进行抗叶锈基因的分子检测。【结果】河农6425 和河农9206 含有抗病基因Lr1 和Lr26，河 农825、河农826、河农827、河农6049 和河农6251 含有Lr26。河农58-3、河农5290、河农6251 和河农825 对 大部分供试菌株表现抗性，可能含有测试基因以外的或未知抗叶锈基因。【结论】河农58-3、河农5290 和河农6251 具有抗叶锈病应用潜力。 关键词：小麦叶锈病；基因推导；抗病性鉴定；分子辅助选择 Evaluation of Wheat Leaf Rust Resistance of 10 New Wheat Cultivars (Lines) SHI Li-hong, ZHANG Na, HU Ya-ya, YANG Wen-xiang, LIU Da-qun (Laboratory of Molecular Phytopathology, Department of Plant Pathology, Agricu ltural University of Hebei/Biological Control Centre of Plant Diseases and Pests of Hebei Province, Baoding 071001, Hebei) Abstract: 【Objective 】The objective of this study was to investigate the leaf rust resistance of 10 new wheat cultivars (lines) including Henong 5290, Henong 58-3, Henong 825, Henong 826, Henong 827, Henong 6049, Henong 6251, Henong 6425, Henong 7106 and Henong 9206. 【Method 】Sixteen Puccinia triticina strains with different virulence patterns were used for seedling genes postulation and 6 strains of them for the test at adult stage. A series of STS and SCAR markers linked to 15 wheat leaf rust resistance genes were used for marker assisted selection of these tested cultivars (lines). 【Result 】Lr1 was found in cultivars Henong 6425 and Henong 9206. Lr26 was detected in 7 cultivars including Henong 825, Henong 826,