退变颈椎间盘组织中细胞凋亡和死亡受体DR5表达的研究.pdfVIP

退变颈椎间盘组织中细胞凋亡及死亡受体DR5表达的研究 王庆锋1锁志刚1丁惠强2 宁夏医科大学 750004银川市 [摘要]目的：探讨死亡受体5(deathreceptor5，DR5)在颈椎间盘退变中的作用。方法：采用原位DNA片 段末端标记(TUNEL)法和免役组织化学方法，对比观察30例颈椎病退变颈椎间盘组织细胞及30例刨伤对 照颈椎间盘组织细胞凋亡状态及死亡受体DR5的表达。结果：TUNEL染色观察示实验组的髓核内的平均 凋亡细胞率为10.93％±1.87％，高于对照组的6.43％±1.56％ （P＜ 0.01）。SP免疫组织化学染色示实验组 的髓核内DR5染色阳性细胞率为50.73％±15.92％,高于对照组41.23％±16.35％,差异有统计学意义(P＜ 0.05)。将实验组和对照组全部60例颈椎间盘标本合并后进行Pearson相关分析，颈椎间盘髓核内DR5染 色阳性细胞率与TUNEL阳性细胞率之间呈正相关，相关系数r为0.755（P＜ 0.01）。结论：高水平的DR5 表达参与了退变颈椎间盘髓核细胞的凋亡，由DR5/TRAIL诱导的凋亡途径可能在颈椎病颈椎间盘退行性 变发生和发展中发挥重要作用。 [关键词]颈椎间盘；退行性变；细胞凋亡；DR5 ThestudiesonexpressionofDR5inthepulpynucleustissueofhumancervicaldiscandtheapootosisofnuclei pulposus WANGQing-Feng1SUOZhi-Gang1DINGHui-Qiang2 1NingxiaMedicalUninersity,Yinchuan750004,China,2DepartmentofOrthopedics,TheAffiliatedHospitalof NingxiaMedicalUninersity,Yinchuan750004,China [Abstract] OBJECTIVE:Toexploretheexpressionofdeathreceptor5(DR5)indegenerativecervicaldisctissue. METHODS:WedetectedtheapoptoticstatusandtheexpressionofDR5in30caseaofdegenerativecervical intervertebaldisctissuesand30oftraumaticnormalcervicalintervertebaldisctissueswithTdT-mediateddUTP biotinnickendlabeling(TUNEL)andimmunohistochemistrymethods.RESULTS:TThepositivityrateofTUNEL stainingwashigherinpatientgroup(10.93％ ± 1.87％)thanincontrolgroup(6.43％ ± 1.56％)(P＜ 0.01).Thepositive expressionrateofDR5ofnucleuspulposusinpatientgroup(50.73％ ± 15.92％)washigherthanthatincontrolgroup (41.23％ ± 16.35％)(P＜ 0.05).Whenthetotal60specimensinthetwogroupswereanalyzed,TheDR5positive incidenceofnucleuspulposusshowedsignificantpositivecorrelationwiththeTUNELpositiveincidenceofnucleus pulposus(r=0.755,P＜0.01).CONCLUSION:HighlevelsofDR5expressionindegenerativecervicalintervertebal disctissuesinvolvedinapoptosis.TheapoptosispathwayinducedbyDR5/tumornecroticfactor-related