退变颈椎间盘组织中细胞凋亡和TRAIL表达的研究.pdfVIP

退变颈椎间盘组织中细胞凋亡及TRAIL表达的研究 锁志刚1王庆锋1丁惠强2 1宁夏医科大学，宁夏，银川 2宁夏医科大学附属医院骨科，宁夏，银川 [摘要]目的：探讨肿瘤坏死因子相关凋亡诱导配体(tumornecroticfactor-relatedapoptosis-inducing ligand,TRAIL)在颈椎间盘退变中的作用。方法：采用原位DNA片段末端标记 （TUNEL)去和免役组织化学 方法，对比观察30例颈椎病退变颈椎间盘组织细胞及30例创伤对照颈椎间盘组织细胞凋亡状态及TRAIL 的表达。结果：TUNEL染色观察示实验组的髓核内的平均凋亡细胞率为10.93％±1.87％，高于对照组的 6.43％±1.56％ （P＜0.01）。SP免疫组织化学染色示实验组髓核内TRAIL染色阳性细胞率为49.13％± 15.66％,高于对照组的20.26±2.81，差异有统计学意义 （P＜ 0.01）。将对照组和患者组全部60例颈椎同 盘标本合并后进行Pearson相关分析’颈椎间盘髓核内TRAIL染色阳性细胞率与TUNEL阳性细胞率之间呈 正相关，相关系数r为0.921(P＜ 0.01)。结论：高水平的TRAIL表达参与了退变颈椎间盘细胞的凋亡， 由TRAIL及其受体诱导的凋亡途径可能在颈椎病颈椎间盘退行性变发生和发展中发挥重要作用。 [关键词]颈椎间盘；退行性变；细胞凋亡；TRAIL SUOZhi-Gang1WANGQing-Feng1DINGHui-Qiang2 1NingxiaMedicalUninersity,Yinchuan750004,China,2DepartmentofOrthopedics,TheAffiliatedHospitalof NingxiaMedicalUninersity,Yinchuan750004,China [Abstract] OBJECTIVE:Toexploretheexpressionoftumornecroticfactor-relatedapoptosis-inducingligand (TRAIL)indegenerativecervicaldisctissue.METHODS: Wedetectedtheapoptoticstatusandtheexpressionof TRAILin30casesofdegenerativecervicalintervertebaldisctissuesand30oftraumaticnormalcervical intervertebaldisctissueswithTdT-mediateddUTP biotinnickendlabeling(TUNEL)and immunohistochemistrymethos.RESULTS:ThepositivityrateofTUNELstainingwashigherinpatient group(10.93％ ± 1.87％)thanincontrolgroup(6.43％ ± 1.56％)(P＜ 0.01).ThepositiveexpressionrateofTRAILof nucleuspulposusinpatientgroup(49.13％ ± 15.66％)washigherthanthatincontrolgroup(20.26± 2.81)(P＜ 0.01). Whenthetotal60specimensinthetwogroupswereanalyzed,theTRAILpositiveincidenceofnucleuspolposus showedsignificantpositivecorrelationwiththeTUNELpositiveincidenceofnucleuspulposus(r=0.921,P＜ 0.01). CONCLUSION:HighlevelsofTRAILexpressionindegenerativecervicalintervertebaldisctissuescanleadto apoptosis.TheapoptosispathwayinducedbyTRAIL/TRAILRmaybeplayanimportantroleintheoccurrenceand progressionofcervicalintervertebaldiscdegeneration. [Keywords] Cervicalinterverteebaldisc;,Degeneration;Apoptosis;TRAIL