HPLC-超滤离心法测定连翘酯苷复方脂质体包封率+.pdfVIP

HPLC-超滤离心法测定连翘酯苷复方脂质体包封率 祝文琪 徐卫康 吴国娟* (北京农学院动物科学技术学院 102206) 【摘要】目的：建立一种快速、准确测定连翘酯苷复方脂质体包封率的方法。方法：采用逆 向蒸发法制备连翘酯苷复方脂质体，建立检测连翘酯苷复方含量的高效液相色谱条件，用超 滤离心法处理复方脂质体，测定连翘酯苷复方脂质体的包封率。结果：用连翘酯苷和绿原酸 标准品建立的标准曲线，表明在0.78125 μg·ml-1-50 μg·ml-1 范围内线性关系良好，采用超滤 离心法处理样品后连续3 次测定包封率，其RSD ≤3% 。结论：HPLC-超滤离心法可准确、 方便的测定连翘酯苷复方脂质体的包封率。 【关键词】：超滤离心法；高效液相色谱法；复方脂质体；包封率 Determination of Entrapment Efficiency of Forsythoside Compound Lipsome by HPLC-Ulrafiltration Zhu Wenqi Xu Weikang Wu Guojuan (Department of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China) [Abstract] Objective: A method for determing entrapment efficiency of forsythoside compound lipsome was established. Method:The lipsome were prepared by reverse evaporation method,and lipsome were treated with ultrafiltration,the method which high performance liquid chromatogra- phy (HPLC) detected the content of drug was assessmented and used for determining the contnet of drug in the forsythoside compound lipsome. Result: The drug can escaped from the lipsome efficiency,and the mean value entrapment efficiency of forsythoside and chlorgenic acid in the thr- ee lot samples were 92.38% and 44.72% with RSD of 2.76% and 2.35%.Conclusion:The entrap- ment efficiency of forsythoside compound lipsome can be determined simply and quikly by the HPLC-Ultrafiltration., [Key words] Ultrafiltration; HPLC; forsythoside compound lipsome ；entrapment efficiency 此文章中连翘酯苷复方是由连翘酯苷和绿原酸两种中药有效成分组成，据研究表明，连 翘酯苷和绿原酸都具有抗炎、杀菌、抗病毒作用[1,2]，将其包裹在脂质体中，可在动物体内 起到缓释作用。脂质体是磷脂分散在水中形成的一个类球形的、包封一部分水相的封闭囊泡， 其包封药物作为缓释制剂是从 1934 年开始[3]，目前国内外载药脂质体技术已经趋于成熟， 并有很多已经应用于临床的药物上市。 [4] [5] 测定脂质体包封率的方法有鱼精蛋白聚集法 、微柱离心法 、透析与反透析法、超滤 离心法等，可根据实际条件和药物的性质等选择合适的方法进行测定，此文结合高效液相色 谱分析技术，采用超滤离心法测定包封率，此法适用于连翘酯苷等水溶性药物[6]，且超滤离 心管的容量可调整，操作方便、快速。 1 仪器、材料与试剂 1