香蕉枯萎病拮抗放线菌Da04010的筛选、鉴定及活性物质初探.pdfVIP

微生物实用技术生态环境应用学术研讨会 香蕉枯萎病拮抗放线菌Da04010的筛选、鉴定 及活性物质初探 叶建军1’2张桂兴1方哲2黄惠琴2鲍时翔2★ (1．海南大学，海u570228；2冲国热带农业科学院热带生物技术研究所，海口571101) 摘要从海南近海海绵、红树林及原始森林采集土样，分离纯化获得271株放线菌，经初筛、复筛和 遗传稳定性试验后，得到一株抗香蕉枯萎病活性稳定的放线菌Da04010，经形态特征、生理生化以及16S rDNA序列分析，分别鉴定为兰灰链霉菌(Streptomycescyaneogriseus)。对其活性物质进行了初步研究， 持较为稳定。 关键词香蕉枯萎病 拈抗菌 生物防治 Studieson andthebioactivematerialof screening，identification antagonistic strainDa04010 Fusariumwiltdiseasesofbanana actinomycetes against Ye ZhF Bao Jianjun’·2ZhangGuixin92Fang HuangHuiqin2Shixian92· (，．Hainan Bioscienceand TAS, University．Haikou570228；2Jnstimte Biotechnology,CA ofTropical Haikou571101) AbstractAtotalof271 strainswereobtainedfrom forestsand forestsinHainan． actinomyces mangrove virgin Astrainofanti—bananawiltdiseaseandactiveness wereobtainedafter and stabilizing passedthroughprimary lestaswellasthe test all strains．Onthebasisof from secondaryscreening hereditystability actinomyces the of1 rDNA characterizationand 6S morphological，Physiological-biochemicalanalysis fragment， sequence thestrain DA04010wasidentifiedas haveconductedthe preliminary Streptomyces．cyaneogriseus．Then，We to toactivemateria