异甘草酸镁论文(仁济医院,陆伦根).pdfVIP

异甘草酸镁对肝细胞体外增殖影响的实验研究 陈尉华 陆伦根 曾民德 刘梅 茅益民 曹爱平 窦爱霞 房静远 【摘要】 目的：观察异甘草酸镁对肝细胞增殖的影响。方法：用含不同浓度异甘草酸镁 的培养基（0、0.001、0.01、0.1、1.0、10.0、100.0mg/ml）培养肝细胞，通过绘制细胞生长 曲线和四甲基偶氮唑盐法（MTT 法）检测异甘草酸镁对肝细胞增殖的影响，并同时观察其 形态变化。结果：在所设加样量范围内，与空白对照组（0mg/ml ）比较，培养基所含的异 甘草酸镁浓度为 1.0mg/ml 时，对张氏肝细胞具有明显的促进增殖作用，异甘草酸镁浓度为 10.0mg/ml 时，其促增殖作用达到最高峰，在异甘草酸镁浓度为 100.0mg/ml 时，则出现明显 的抑制作用。结论：异甘草酸镁对肝细胞的增殖具有明显的影响，在一定剂量范围内对肝细 胞具有明显的促进增殖作用。 【关键词】异甘草酸镁；肝细胞；增殖 Effect of magnesium isoglycyrrhizinate on the proliferation of hepatocytes in virto CHEN Wei-hua, LU Lun-gen, ZENG Min-de, LIU Mei, MAO Yi-min, CAO Ai-ping, DOU Ai-xia Departmentment of Gastroenterology, Renji Hospital, Shanghai Institute of Digestive Disease, Shanghai Second Medical University, Shanghai 200001,China. 【 Abstract 】 Objective To investigate the effect of magnesium isoglycyrrhizinate on the proliferation of hepatocytes. Methods Hepatocytes were incubated with various concentrations of magnesium isoglycyrrhizinate solution(0mg/ml, 0.001mg/ml, 0.01mg/ml, 0.1mg/ml, 1mg/ml, 10mg/ml,and 100gm/ml).By making cell growth curve and 3- (4,5-dimethylthiazol-2-yl)-2,5-diphennylterazolium bromide(MTT) colorimetric assay to observe the effect of maganesium isoglycyrrhizinate on the proliferation of hepatocyte in virto, and morphological changes of the hepatocytes were also observed. Results Compared with the control group(0mg/ml group),the proliferation of hepatocytes obviously increased when the concentration of magnesium isoglycyrrhizinate in medium reached 1.0mg/ml within the predefined different dosages range. The proliferation of hepatocytes presented its peak when the magnesium isoglycyrrhizinate’s concentration was 10.0mg/ml. But, the proliferation of hepatocytes was significantly inhibited, when the concentration of magnesium isoglycyrrhizinate in medium