盾叶薯蓣离体繁殖与微块茎诱导的研究.pdfVIP

华中农业大学学士学位论文（设计） 学士学位论文 题 目 盾叶薯蓣离体繁殖与微块茎诱导的研究 姓 名 李琼 学 号 200106024 专 业 园艺 指导教师 姜玲 职 称 副教授 中国·武汉 二○○五年 五月 华中农业大学学士学位论文（设计） 盾叶薯蓣离体繁殖与微块茎诱导的研究* 摘 要 以盾叶薯蓣的茎尖、嫩叶和果荚为外植体建立了以诱导微块茎为目的的离体繁殖体系。试验显 示，茎尖外植体出愈率高，出愈时间短。MS+0.5 mg/L NAA +0.5 mg /L BA 适合于各类外植体愈伤组织诱 导；0.1 mg/L NAA +1.0 mg/L KT 培养基上愈伤组织出芽率最高，平均芽数也最多；0.5 mg/LNAA+2.0 mg/L BA 有利于腋芽增殖。离体再生植株的幼嫩茎段可用来诱导微块茎，结果发现，高浓度 BA和高浓度蔗 糖有利于微块茎的形成，试验中以8.0 mg /L BA +1.0 mg/L KT + 80g/L Suc 为配方的培养基微块茎形成 率最高, 4-5周的暗期处理，有利于微块茎的形成。 关键词 盾叶薯蓣；离体繁殖；微块茎 Research on in vitro micropropatation and microtuber inducing of Dioscorea Zingiberensis C.H Wrihgt Abstract The shoot tip, young leaf and pericarp of Dioscorea zingiberensis C.H Wrihgt were used as explants for establishment of in vitro propagation system with the aim to induce microtubers. The experimental results showed shoot tip was optimum explant due to the high percentage and short time of callus induction. MS+0.5 mg/L NAA +0.5 mg/L BA was optimum for callus induction of all types of explants. The highest percentage of bud differentiation was observed on MS+0.1 mg /L NAA +1.0 mg./L KT associated with the most mean shoot number per callus. MS+0.5 mg /L NAA+2.0 mg /L BA was most efficient for axillary bud multiplication. The shoot segments of regenerated plant were used for induction of microtuber. Research findings showed high-concentration BA and sucrose favored microtuber formation. The highset percentage of microtuber formation was obseved in presence of 8.0 mg /L BA +1.0 mg /L KT + 80g /L Suc.