泛耐药铜绿假单胞菌_内酰胺酶基因分型及菌株亲缘性分析.pdfVIP

2009 19 13 ·1621 · ·　 · β- 1 1 2 糜祖煌 , 秦　玲, 金　辉 (1., 214026;2. , 210009) :　(PDRPA )β-。　PCR 21 β-、。　33 PDRPA blaTEM、blaOXA-10 、blaPER、 blaGES、blaCARB、int Ⅰ1、merA 48.5%、45.5%、33.3%、21.2%、15.2%、 8.8%、69.%; 。　PDRPA blaT EM、blaOXA-10 、blaPER、blaGES、blaCARB、int Ⅰ1、merA , PDRPA 。 :;β-; + :R3 8.99 1　　:A 　　:1005-4529(2009)13-1621-03 Genes ofβ-Lactamase inPan-drugResistantPseudomonas aerug inosa andPhylogeneticAnalysis ＊ M I Zu-huang , QIN Ling, JIN Hui (＊Wux i Clone Gen -Tech I nsti tu te, Wu x i, J iang x u 214026, Ch ina ) Abstract:OBJECTIVETo investigate the presence of β-lactamase genes in pan-drug resistant P s eud om onas aerug i- nosa (PDRPA), and the redateness in PDRPA. ETHODSThe genes of 21 kinds of resistance w ere detected by polymerase chain reaction and phylogenetic analysis.RESULTS In 33 strains of PDRPA, the positive rates of blaTEM, blaOXA-10cluster, blaPER, blaGES, blaCARB, int Ⅰ1 and merA w ere 48.5%, 45.5%, 33.3%, 21.2%, 15.2%, 8.8% and 69.%, respectively.There w ere clone transmitted phenomena.CONCLUSIONS There are very high positive percentages of blaT EM, blaOXA-10cluster, blaPER, blaGES, blaCARB, int Ⅰ1 and merA genes in PDRPA.The PDRPA can induce clone transmitted hospital infection. Keywords:Pan-drug resistant P seud omonas aerug inosa ;β-Lactamase;Phylogenetic analysis [3] 　　 、 ) 。(PDRPA) 、 [1,2] 。 ,。 33 PDRPA 16S rRNA 1　 [3] 、 , PDRPA β-, 1