家蚕蛋白激酶C受体基因Bmrack的克隆和序列分析.pdfVIP

家蚕蛋白激酶C 受体基因Bmrack 的克隆及序列分析 1 1 1 1 1 1 1 1, 2 刘艳艳 池旭娟 方向明 阚雪芹 苏媚 刘文倩 王海玲 谈建中 (1 苏州大学建筑与城市环境学院, 苏州 215123; 2 苏州大学蚕桑研究所, 苏州 215123) 摘 要 蛋白激酶C 受体(receptor for activated protein kinase C，RACK)蛋白属于一类含有WD-40 重复结构蛋白家 [1] [2] [2] 族的亚家族 ，除了作为细胞内蛋白激酶C 的受体 之外，也作为细胞内重要的支架蛋白调节细胞功能 。研究发 [3] [4] 现，RACK 蛋白在小鼠胚胎发育过程中表达 ，可能与胚胎着床及蜕膜化过程密切相关 ，对小鼠受精卵的发育具 [5] 有调控作用 。笔者在家蚕5 龄幼虫和蛹期的脂肪体蛋白质组研究中，发现RACK 蛋白仅在雌蚕的5 龄盛食期表 达，暗示该蛋白对家蚕性别与发育可能具有重要功能。因此，本研究采用生物信息学和分子生物学方法，以5 龄 幼虫脂肪体基因组DNA 为材料，克隆了家蚕RACK 蛋白的Bmrack 基因，并探讨了Bmrack 基因的结构特征和RACK 蛋白的功能，以期为阐明家蚕性别发育及其调控的分子机理提供参考。 Cloning and sequence analysis of Bmrack gene in Silkworm, Bombyx mori LIU Yan-Yan1 CHI Xu-Juan1 FANG Xiang-Ming1 KAN Xue-Qin1 SU Mei1 LIU Wen-Qian1 WANG Hai-Ling1 TAN Jian-Zhong1,2 1 ( School of Architecture and Urban Environment, Soochow University, Suzhou 215123, China; 2 Institute of Sericulture, Soochow University, Suzhou 215123, China) Abstract: Receptor for activated protein kinase C (RACK) was an important protein which was differential expressed between male and female silkworm during developmental stages. So Bmrack gene was in silico cloning based on Bioinformatics and a conting of 1224 bp was obtained. The length of its coding region was 960 bp and coding 319 aa. Its molecular weight was 36 kD and pI was 7.64. Moreover, the amino acid sequences were highly conserved in Lepidotera. After that, Bmrack gene was cloning from silkworm genome DNA on PCR and a sequence with a length of 2122bp was