二苯乙烯苷对阿霉素心脏毒性的保护作用及机制.pdfVIP

维普资讯 中国药理通讯 2008年第二十五卷第二期 receptor4(TL )duringcerebralischemia／reperfusion(C )．Methods：InBV一2microgliacellline， RT—PCRassayandwesternblotnaalysiswereappliedtostudytheexpressionofrIL inmRNA level nadinproteinlevelafterhypoxianadreoxygenation,WealsoobservedhteinfluenceofAngIInadits blokersonhteexpressionof ．To reveal htereceptormechna isriaofAng，hteactionofAT1 receptorblockerlosartna nadAT2rec印torblockerPD123319wereobservedindividually．Fornaalysis oftheroleofAngIIonTUR4expressionafterCIR invivo，Transientfocalcerebralischemiawas producedbyhteMCAO procedureinmalerats．The1f1Rdexpressionpatternswereinvstigatedby RT．PCRassaynadimmunohistochemicalmehtod．Toobserve,hteinfluenceofAngII，someratswere treatde wiht ACEIcaptoprilorAT1receptorblockerlosartan 14daysbeforehteopration．Results：Our studyshowshtatinBV-2microgliacellline，rI1一 wasupregulatde inmRNA nadproteinlevelsafter hypoxiafor2hnadreoxygenationofr6hor12h．AngIIsignificantlyincreasedhypoxiainducedTLIL ． ． expression．Th eupregulationwasalmosttotallyabolishedbyAT1rceeptorblockerlosartan；whereas hteA receptorblockerPD123319hadnoinhibitoryeffect In vivo study also showedhtathte expressionofTLR4mRNA nadTLR4proteinwasupregulatedsignificantlyafterischemiaofr2hnad repeffusionfor2hor6h．Boht ACEIcaptoprilnadATIreceptorblockerlosartancould inhibithte upregulationofrIL expressionafterCR／．Conclusion：AngI1wasinvolvedinhteupregulationof expressionafterCIRviana AT1pahtway． 二苯乙烯苷对阿霉素心脏毒性的保护作用及机制 张韶辉 常慕君 王嘉陵 华中科技大学同济医学院药理学系 武汉 430030 目的I研究中药何首乌主要有效成分二苯乙烯苷 (THSG)对阿霉素(ADM)诱导的心脏毒性 的保护作用及机制。方法：在ADM诱导的小鼠急性心脏损伤模型及急性分离培养的乳大鼠心肌 细胞上，采用形态学、生物化学及分子生物学等方法分别研究了THSG对阿霉素心脏毒性的保 护作用及其机SUo结果：在整体小鼠阿霉素急性心脏损伤模型 THSG (