A new strategy to protect flowers from freezing stress.ppt

A new strategy to protect flowers from freezing stress Liao Pan (廖攀), Chen Qin-Fang (陳琴芳), Chye Mee-Len (蔡美蓮) Wilson Amelia Wong Professor in Plant Biotechnology 黃乾利辛烱僖基金教授席 (植物生物技術學) School of Biological Sciences, The University of Hong Kong /content/early/2014/02/19/pcp.pcu037 * Significance: flowers develop into fruits and seeds (food) Fruit crops Flowers Damaged flowers Protected Freeze-damaged FOOD LOSS ACYL-COA-BINDING PROTEIN6 (ACBP6) (醯基輔酶A結合蛋白6) A cold-inducible protein Expressed in both rosettes and flowers Rosettes = vegetative tissues Flowers = reproductive tissues Chen et al., 2008, Plant Physiol 148: 304-315 Arabidopsis Chye et al (2013) US Patent No. 8,378,172 ACBP6 overexpression in transgenic Arabidopsis (轉基因擬南芥) enhances freezing tolerance in rosettes Rosettes OE = overexpression lines; 3 OE lines were used to test reproducibility of results Freezing treatment on Arabidopsis flowers Flowers Wild type (WT) ACBP6-overexpressors (OE) 4oC 3 days 1 -7oC 1 hour 2 4oC 12 hours 3 4 Harvest flower samples ACBP6-overexpressing flowers are freeze-tolerant Transformed Arabidopsis flower overexpressing ACBP6 (ACBP6-OE) shows intact petals (right) while the control succumbs to freezing stress (left). Liao et al. 2014, Plant Cell Physiol 54% 86% flowers with intact petals (-7oC 1 hour) Why are ACBP6-OE flowers freezing tolerant? PC increases membrane fluidity After freezing treatment, phosphatidylcholine (PC) (卵磷脂) accumulates (25-34% WT) increasing membrane fluidity, enhancing freezing tolerance. Liao et al. 2014, Plant Cell Physiol Liao et al., 2014, Plant Cell Physiol WT OE-3 OE-5 MGDG 29.6 ± 1.33 39.6 ± 0.53 36.0 ± 2.95 Plastid-specific membrane galactolipids (nmol mg-1) 質體特有的光合膜半乳糖脂（納摩爾每毫克） Why are ACBP6-OE flowers freezing tolerant? After freezing treatment, Monogalactosyldiacylglycerol (MGDG) (單半乳糖二醯甘油酯) accumulates [(MGDGOE-MGDGWT)/MGDGWT = 22-34