茶叶及茶多糖中氟测定前处理方法的比较研究.pdfVIP

茶叶科学 2015 ，35 （2 ）：145~ 150 Journal of Tea Science 投稿平台： 茶叶及茶多糖中氟测定前处理方法的比较研究 朱晓静，房峰祥，张月华，倪德江，陈玉琼* 园艺植物生物学教育部重点实验室，华中农业大学园艺林学学院，湖北 武汉 430070 摘要：以不同品种茶树鲜叶及茶多糖为材料，比较了酸浸提、沸水浸提和碱熔灰化前处理对氟离子选择电极 法测定氟含量的影响。结果表明，无论对茶叶还是茶多糖，碱熔灰化处理所测氟含量均极显著高于酸浸提和 沸水浸提法 （P0.01 ）。茶叶水浸提法氟含量显著或极显著高于酸浸提法 （P0.05 ，P0.01 ）。对碱熔灰化- 氟离子电极法进行精密度及回收率实验，结果表明茶叶和多糖中氟的回收率分别达到 91.07%~94.40% 和 83.04%~90.32% ，而RSD 分别为1.44%~2.54%和0.68%~ 1.03% ，说明该方法稳定性好，精密度高，检测结果可 靠，更能真实反映茶叶及茶多糖的氟含量，适宜于茶叶及茶提取物全氟的测定。 关键词：茶叶；茶多糖；氟含量；预处理方法；氟离子选择电极法 中图分类号：S571.1 ；O652 文献标识码：A 文章编号：1000-369X （2015 ）02- 145-06 Comparison on the Pretreatment Methods of Tea and Tea Polysaccharides for Determination of Fluorine Content * ZHU Xiaojing, FANG Fengxiang, ZHANG Yuehua, NI Dejiang, CHEN Yuqiong Key Laboratory of Horticultural Plant Biology , Ministry of Education/College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China Abstract: The influence of sample pretreatment methods including boiling water extraction (BWE) ，acidic solution extraction (ASE) and alkalizing and burning to ash(ABA) on fluorine content in tea and tea polysaccharides was investigated. The results showed that the fluorine contents of tea and tea polysaccharide pretreated with ABA were much higher than that of BWE and ASE method (P0.01) . The fluorine content of tea pretreated with BWE was significantly higher than ASE (P0.05 , P 0.01). The further test on precision and recovery of ABA and fluoride ion selective electrode method was investigated . Results showed that the recovery of fluorine in tea and tea polysaccharide were 91.07%