新型离子交换型晶胶介质的制备及性能研究.pdfVIP

新型离子交换型晶胶介质的制备及性能研究 陈艳，沈绍传，贠军贤，姚克俭1 （绿色化学合成技术国家重点实验室培育基地浙江工业大学化学工程与材料学院，浙江 杭州310032 ） 摘要：离子交换超大孔连续床晶胶介质作为一种新型分离介质具有良好的选择性，传质阻力小，吸附分离 迅速等优点。本研究中将带有双羧基功能团的衣康酸单体固载到丙烯酰胺骨架上，研究其接枝前后基本性 能的变化，并以溶菌酶为模型蛋白,考察了其在两次接枝后晶胶介质内穿透曲线及吸附容量，以表征蛋白质 在此晶胶介质内的吸附、解吸特性；同时也考察了不同流速对其吸附特性的影响。 关键词：超大孔连续床；离子交换；衣康酸；溶菌酶；吸附；解吸 中图分类号： 文献标识码： Preparation and characteristics of a novel anion-exchange continuous supermacroporous cryogel CHEN Yan， SHEN Shao-chuan ，YUN Jun-xian ，YAO Ke-jian (State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China) Abstract ：The anion-exchange continuous supermacroporous cryogel is a novel separating medium, which permits high-selectivity, low back pressure to mass and quick isolating biomolecules. In this work, the polyacrylamide-based cryogel matrix was grafted with itaconic acid. The study compared characteristics of the second grafted cryogel with the first grafted cryogel. In order to reveal the protein adsorption characteristics in this novel cryogel, lysozyme was used as model protein to study the adsorption and desorption behaviors of the cryogel. The breakthrough curves of lysozyme and the adsorption capacity of the cryogel were obtained and the effect of different flow velocities to the cryogel was investigated. Keywords: Continuous supermacroporous cryogel; Anion-exchange; Itaconic acid; Lysozyme; Adsorption; Desorption 联系人：姚克俭，电话：0571，E-mail ：yaokj@zjut.edu.cn 第一作者：陈艳（1982-），女，湖南益阳人，浙江工业大学硕士研究生。 基金项目：浙江省自然科学基金项目（No. Y405054 ，Y407366 ），浙江工大东晖科技创新基金项目。 1.2.2 双羧基功能单体的固载 引 言