生物医药论文-雷公藤药材及制剂的HPLC指纹图谱研究.doc

雷公藤药材及制剂的HPLC指纹图谱研究 摘要 目的：建立药材的HPLC指纹对照测定方法：采用C18色谱柱甲醇-梯度洗脱药材指纹图谱共有模式以最接近共有模式的药材为指纹对照采用夹角余弦法分别从化学成分和含量两个方面对不同产地药材的HPLC指纹图谱进行相似性评价。结果：建立的药材指纹图谱分离好结论：以指纹对照品药材良好药材关键词：雷公藤高效液相色谱法 Tripterygium wilfordii Hook. f and tripterygium tablets by HPLC Fingerprint Chromatogram Abstract Objective: To establish a stable and repeatable HPLC reference fingerprint chromatogram standard for the quality analysis and control of Tripterygium wilfordii Hook. f. and its preparations. Methods: HPLC separation was performed on a C18 column with methanol-water as mobile phase in a gradient elution mode. The reference fingerprint chromatogram was determined as the most typical chromatogram that represented most properties of Tripterygium wilfordii Hook. f. and tripterygium tablets, respectively, from both constitutes and quantity view of points, established by statistical and similarity analyses. Results: The results indicate that the quality assessments of Tripterygium wilfordii Hook. f. and tripterygium tablets with reference to the concomitantly measured HPLC reference fingerprint chromatograms have good ruggedness, specificity, and reproducibility. Conclusion: The fingerprint chromatograms of Tripterygium wilfordii Hook. f. and its preparations can be quantitatively and qualitatively evaluated by comparison with the established fingerprint reference standards by using Cosine ( and relative Euclid distance methods, respectively. The fingerprint chromatograms of both Tripterygium wilfordii Hook. f. and its preparations varies significantly. Key words: Tripterygium wilfordii Hook. f.; tripterygium tablets; triptolide; fingerprint chromatogram; HPLC 雷公藤(Tripterygium ilfordii Hook. f.)系卫矛科雷公藤属植物，Tripterygium hypoglaucum Hutch)、东北雷公藤(Tripterygium regelii Sprague et Takeda)。雷公藤是一味传统中药，具有抗炎、免疫抑制、抗肿瘤、抗生育等活性[1]，这些作用是由其所含的二萜和三萜内酯、及生物碱等多种成分的协同作用所产生的[2]。雷公藤多苷是从卫矛科植物雷公藤根中提取出来的一些次代谢物的混合体,具有抗炎和免疫抑制作用，在临床上雷公藤多苷片并在抗肿瘤方面具有前景[]，其抗生育[5]。[6]。 尽管已有大量的雷公藤化学成分的分离鉴定研究和雷公藤内酯(雷公藤多苷)的药理研究报道[2,6~9]