梨种质资源SSR引物筛选和评价.pdfVIP

12 梨种质资源SSR 引物筛选与评价 许靖诗 1,2，乌云塔娜 1,2 1. 中南林业科技大学 经济林培育与保护教育部重点实验室，湖南长沙 410004 ；2. 中南林业科技大学 经 济林栽培与育种国家林业局重点实验室，湖南长沙 410004 摘要：目前在梨种质研究中仍然缺乏足够的SSR 引物，本研究筛选出多态性丰富的 SSR 标 记引物，为进一步研究梨种质资源的群体遗传学特征等提供参考。运用 SSR 标记技术，从 初选后的 42 对 SSR 引物中筛选出多态性好、稳定性高的引物，筛选出的 12 对引物对 40 个 梨样的 DNA 序列进行了 SSR-PCR 扩增, 共检测出 159 个等位基因，平均每个位点检测到 13.25个等位基因;平均每个等位基因有效数目为 1.236 4,平均I 值为0.286 6，平均h 值为0.165 3;系统聚类分析表明，所有样品可以分为 3 个类群，即栽培梨、日本梨、野生秋子梨。筛选 的 12 对多态性丰富的 SSR 引物适合用于梨种质资源群体遗传学特征分析，为梨种质资源的 收集、保存、鉴定、评价以及亲缘关系研究提供方法和理论依据。 关键词：梨，SSR，引物筛选 SSR primer screening and assessment on Pear germplasm resources XU Jing-shia,b ,WUYUN Ta-naa,b (a. Key Lab of Non-wood Forest Product of Forestry Ministry; b.School of Forestry , Central South University of Forestry and Technology, Changsha 410004,Hunan,China) Abstract: In present study, lacking enough SSR primers in the pear germplasm study, this study selected SSR markers primer showing high polymorphismin for further research on population genetic characteristics. Good polymorphism and high stability primers selected from the 42 pairs of SSR primers by SSR molecular primer .The 40 pear samples were analyzed by 12 pairs of SSR primers ,159 alleles were detected, on average, 13.25 alleles for each site . Each alleles effective number was 1.236 4, and the average I value was 0.286 6, the h value was 0.165 3. The system clustering analysis showed that all the samples can be divided into three groups: cultivation pear, the Japanese pear, Pyrus ussuriensis Maxim in China. The screened 12 pairs of polymorphic SSR primers were suitable for studying on collection, preservation, identification, evaluation and genetic relationship of pear germplasm resources . Keywords: pear; SSR molecular markers; primer screening 梨为蔷薇科 Rosaceae 梨亚科 Romoideae 梨属 Pyrus多年生果树，我国为梨属植物 的发祥地, 境内蕴藏着丰富的梨