A Perfusion-based Micro 3-D Cell Culture Platform.ppt

A Perfusion-based  Micro 3-D Cell Culture Platform NTHU MSCL Adviser: C. H. Liu C. He Yeh A Perfusion-based  Micro 3-D Cell Culture Platform Outline Culture environment at large scale Culture environment at micro scale Principle Fabrication Conclusion Features Further work General Culture Equipment 細胞培養設備 (1) 高溫高壓滅菌爐 (2) 無菌室 (3) 無菌操作台 (4) 二氧化碳培養箱 (5) 細胞離心機 (6) 倒立顯微鏡 (7) 恆溫水浴槽 (8) 冷凍冷藏冰箱 (9) 細胞計數器及血球計數盤 (10) 本生燈 (11) 液氮細胞儲存桶 (12) 酸鹼測定儀 (13) 真空幫浦 (14) 瓦斯桶 (15) 二氧化碳桶 On Chip Advantages Small dimension Saving sample Rapid assay process (micro dimension, avoid medium or reagent diffusion time gradient or concentration different ) Cheap, disposable On Chip Culture Requirement Temperature : 37℃ pH : 7.2 ~ 7.4 ( 95% air 5%CO2 or HEPES) Buffered saline (PBS, PBSA, …etc) Medium : (Serum, glucose, amino acids, vitamin, antibiotics ) 3D culture (In vivo Culture) A Perfusion-based  Micro 3-D Cell Culture Platform Working Principle : A Perfusion-based  Micro 3-D Cell Culture Platform Fabrication : Conclusion Feature 1: 3D well-defined culture environment mimic an in vivo environment distinct cellular behaviors (cell-cell and cell-extracellular interactions ) provide more meaningful data Conclusion Feature 2 : Short Loading cell channel Feature 3 : High throughput (8 chambers) Further work Bioassay (inject reagent instead of culture medium in vivo bioassay) Biochemical experiment Tissue engineering Drug screening μTAS Reference “A PERFUSION-BASEDMICRO 3-D CELL CULTURE PLATFORM” S. B. Huang1, M. H. Wu2, Z. F. Cui3, Z. Cui2, J. L. Lin4 and G. B. Lee1* “MICROFLUIDIC DEVICES FOR THE ASSEMBLY AND CULTURE OF THREE-DIMENSIONAL MULTI- CELLULAR CONSTRUCTS WITH DIFFUSION-LIMITED MICROENVIRONMENTS” M. Pinelis1, R. W. Kasinskas2, R. T. Borno1, J. H. Park1, E. Chu3, N. S. Forbes2 and M. M. Maharbiz1 Differentiation of human Embryonic Stem Cells into Hepatocytes in 2D and 3D cultur