常见呼吸道病毒分子鉴别诊断技术的建立.pdfVIP

20066 20 2 Chinese J Exp Clin Virol,June 2006, Vol 20, No12 # 17 # ## 邹淑梅 韩健 温乐英 assi Cronin 舒跃龙 = , RT-PCR 13 13 , 100%;10e2-10e1(pfuPml) , = ; Establishment of molecular differential diagnostic (MDD) technology for detection of common respiratory * * viruses ZOU Shu-mei , HAN j ian, WEN Le-ying , Kassi Cronin , SH U Yue-long . State Key Laboratory f or I nf e tious Disease Prevention and Control , Institutef or Viral Disease Control and Prevention, Chinese Center f or Disease Control and Prevention, Beij ing 100052, China Corresponding author : SHU Yue-long , E-mail :yshu@vip . sina. om.Tel :010 = Abstract Objective To provide rapid laboratory evidence for diagnosis of respiratory infection and help diagnose accurately and reduce the spread of disease, so that the patients can be diagnosed and treated early. Methods Thirteen kinds of respiratory viruses were detected by using the Genacops MDD technology. Results All the specimens were detected, the total positive ratewas 100%; the sensitivity of the method was 10e2( pfuP ml) . Conclusion The MDD system can distinguish the 13respiratory viruses, which helps diagnosis of respiratory viral infection. = Key ords Molecular differential diagnostic; Respiratory virus , 90% MDD, [ 1] , 1 , PCR 3 ; 13 (Target enriched multiplex PCR, Tem-PCR) Multiple (RSV)A B [2] Analytes Profiling(xMAP) , , PCR , , , , , , , 1997 1 2003 SARS ,, 111