人类A组轮状病毒Vp4的表达与免疫反应性.pdfVIP

VIROLOGICA SINICA, August 2007, 22 (4):287-293 CLC number: Q786 Document code: A Article ID: 1003-5125(2007)04-0287-07 Expression and Immunoreactivity of a Human Group A Rotavirus Vp4 Qing-huan ZHAO, Yu-ling WEN, Yang YU, Qing DAI and Yuan-ding CHEN* （Institute of Medical Biology of Chinese Academy Medical Sciences Peking Union Medical College, Kunming 650118, China ） Abstract: Rotavirus capsid protein Vp4 plays an important role in the virus adhering and entering the cells. In this study, a Vp4 gene cloned from a rotavirus strain TB-Chen was highly expressed in E.coli BL21 (DE3). The results of the Western blot showed that the protein possesses specific immuno-reactivities and can be specifically recognized by guinea pig antibodies against rotavirus strain SA11 or Wa. Some Vp4 dimers were formed during renaturation. These data obtained from this study provide a strong basis for further study on the structure and function of the Vp4. Key words: Recombinant rotavirus VP4; Prokaryotic expression; Immunoreactivity. Rotavirus (RV), belonging to the family of Reoviridae, The Vp4 can be cleaved into Vp5* and Vp8* by the is the leading cause of severe viral gastroenteritis in trypsin-like proteases, and virus infectivity is enhan- infants and children worldwide. In developing countries ced by this cleavage (6). From the above discussion, it RV infections cause approximately 600,000 children is clear there are several theoretical and practical deaths per year(15). In addition, rotaviruses also infect benefits to further study of this protein. This study mammals and poultry. Therefore, RV not only threaten reports the results of the gene cloning, recombi- nant human health but also impact the development of the