茶叶中EGCG对小鼠抗凝血作用实验研究StudyontheEGCG.pdfVIP

茶叶科学 20 11，31 （6 ）：532~536 Journal of Tea Science 茶叶中EGCG 对小鼠抗凝血作用实验研究 1 2 1 1 王贤波 ，成浩 ，赵芸 ，黄海涛 1. 杭州市农业科学研究院实验中心，浙江 杭州 310024 ； 2 . 国家茶树改良中心，中国农业科学院茶叶研究所，浙江 杭州 310008 摘要：以ICR 小鼠为模型，对小鼠抗凝血指标进行测定。实验表明，含EGCG 0.25 、0.5 、1.0 g/(kg ·d)剂量组 与对照组比较能极显著延长小鼠尾出血时间（BT ）和活化部分凝血活酶时间（APTT ），显著降低纤维蛋白原 （FIB ）含量，对小鼠凝血酶原时间（PT ）没有影响。含 0.5 、1.0 g/(kg ·d)EGCG 剂量组与对照组比较能显著 延长小鼠凝血时间（CT ）。由试验结果推测 EGCG 能抑制内源性凝血途径，延长凝血时间，具有较好的抗凝 血作用。 关键词：茶叶；EGCG ；ICR 小鼠；抗凝血 中图分类号：TS272 ；R973+ .2 文献标识码：A 文章编号：1000-369X （2011 ）06-532-05 Study on the EGCG from Tea on the Blood Anticoagulation Effect of Mice 1 2 1 1 WANG Xian-bo , CHENG Hao , ZHAO Yun , HUANG Hai-tao 1. Laboratorial center ,Hangzhou Institute of Agricultural Sciences, Hangzhou 310024, China ； 2 . National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 3 10008, China Abstract : The effect of EGCG on blood anticoagulation of mice was evaluated in this investigation. Results showed that EGCG with doses of 0.25 g/(kg·d), 0.5 g/(kg·d) and 1.0 g/(kg ·d)could significantly prolong bleeding time of mice tail and activated the partial thromboplastin time compared with control group, effectively depress fibrinogen level, while EGCG nearly has no impact on mice prothrombin time . EGCG with doses of 0.5 g/(kg·d) and 1.0 g/(kg ·d) could effectively prolong coagulation time compared with control group . It is calculated that EGCG could inhibit the intrinsic coagulation pathway and prolong the blood coagulation time . EGCG showed obvious blood anticoagulation eff