四、GeneRegulation.ppt

?组蛋白的修饰 ?组蛋白的修饰改变染色质的结构，直接或间接 影响转录活性（磷酸化、甲基化、乙酰化，泛素化（uH2A） // Arg，His，Lys，Ser，Thr） ?组蛋白赖氨酸残基乙酰基化（acetylation），影响转录 ?HMG结构域蛋白等染色质变构因子的影响 ?HMG结构域可识别某些异型的DNA结构，与DNA 弯折和DNA-蛋白质复合体高级结构的形成有关 Regulation of Untranslated Regions of mRNA 1. Scan model（SD Sequence） 2. Methylated cap(m7?G) of mRNA N N A U G G A G 3. Stability of mRNA 4. Modification of translation factors Gene Regulation of Eukaryote —— Regulation of translation 1. Processing S S S S S S Gene Regulation of Eukaryote —— Regulation of protein S S S S S S S S S S S S 1. Processing Gene Regulation of Eukaryote —— Regulation of protein 1. Processing Gene Regulation of Eukaryote —— Regulation of protein 2. Modification (methylation，acylation) 3. Glycosylation 1. Processing Gene Regulation of Eukaryote —— Regulation of protein cis-acting element of Gene silencer transposable element promoter enhancer promoter -- 近端顺式元件: 核心启动子和上游启动子元件(UPE) enhancer -- 远端顺式元件: 特点和作用机制 典型启动子：TATA box 不典型启动子：a. GC rich b. No TATA box and GC box GB14基因上游启动子预测结果 -518 -418 -318 -218 -118 -18 cis-acting element of Gene OPB7-1基因的结构分析 OPB7-1b OPB7-1a DNA FLJ 20508 BC018109 isoform E 1 E 1 E 1 E 1 Promoter 1 Promoter 1 Promoter 1 Promoter 2 C3 C2 C1 T C1:CAATCC (－124bp～－127bp ) 生物信息学分析结果 顺式作用元件 经典序列 变异序列 TATA box TATA(A/T)A (A/T) T:TATATTT(－45bp～－48bp ) CAAT box CAATCA C2:CAATtA (－185bp～－188bp ) C3:CAATgt (－275bp～－278bp ) GC box GGCGGG GGCGGG(－400bp～－2000bp) Gene expression is regulated --transcriptional level, translation level Concept in regulation of gene cis-acting element trans-acting factor structural gene ——encoding protein or RNA that basic component of cell or tissue regulator gene ——encoding protein or RNA that be concernd with regulation of other gene expression regulator gene control transcription by binding specific site of DNA Comparability between Eukaryote and Prokaryotic interaction * * Regulation of Gene Expressi