高效液相色谱法测定唑来膦酸中的磷酸盐和亚磷酸盐_马仕珉.pdfVIP

19 4 ( ) Vo l 19 No 4 20 10 12 J ournal o f H uaih ai Institute o f T echno log y( N at ural S cien ce Edit ion) Dec 2010 DO I: 10 3969/ j issn 1672- 66 85 20 10 04 011 * 马仕珉, 石松安, 季晶晶, 李语如 ( , 222002) : 建立了测定唑来膦酸中磷酸盐和亚磷酸盐的高效液相色 法以IC- PA K 色 柱为固定 相, 以体积分数0107 % 的甲酸溶液( 用 1 mol/ L 氢氧化钠溶液调节pH 至315) 为流动相, 采用折光 检测器检测唑来膦酸样品唑来膦酸磷酸盐和亚磷酸盐3 组分分离完全, 检测限分别为15, 20 和 15 ng, 线性关系良好, 相对标准偏差均小于1% 该方法简便准确, 适用于唑来膦酸中磷酸盐和亚 磷酸盐的测定 : 高效液相色 法; 唑来膦酸; 磷酸盐; 亚磷酸盐 : O657 : A : 167 2- 6685( 2010) 04- 0040- 03 Determination of Phosphate and Phosphite in Zoledronic Acid by HPLC MA Shi- min, SH I Song- an, JI Jing-jing, L I Y u- ru ( J iangsu H eng ru i M edicine Co , Lt d , L ianyungang 222002, China) Abstract: A new analy tical met ho d based on high p erfo rmance liqu id chrom at og raphy ( H PL C) w as obt ained f or t he det erminat ion of pho sphat e and phosphit e in zo ledronic acid T he chromat o- graphic sep aration of zoledronic acid sample w as achiev ed on an IC- PA K co lum n w it h a refr act iv e index det ect or T he mo bile ph ase w as com po sed of 0 07 % f ormic acid and t he pH w as adju st ed t o 3 5 w it h 1 mol/ L so dium hy dro xide so lut ion Zoledr onic acid , phosph at e and phosphite w er e complet ely sep arat ed and t he det ection lim it s w er e 15 ng, 20 ng and 15 ng r espect ively T he cali- br at ion cur ves show ed g ood linearit y, and t he relative st andar d deviat ion s ( RS D ) w er e less t h an 1% T he met