表没食子儿茶素没食子酸酯甲基化分子修饰.pdfVIP

※基础研究 食品科学 2010, Vol. 31, No. 15 139 表没食子儿茶素没食子酸酯的甲基化分子修饰 吕海鹏，孙业良，林 智* ，谭俊峰，郭 丽 ( 中国农业科学院茶叶研究所，茶叶加工工程研究中心，浙江杭州 3 10008) 摘 要：研究表没食子儿茶素没食子酸酯(EGCG) 的甲基化分子修饰。以碘甲烷作为甲基供体，采用化学合成方法 研究EGCG 的甲基化分子修饰，并通过HPLC-M S 和N MR 等对反应产物进行结构鉴定。结果表明：采用化学合 成方法能有效完成E G C G 的甲基化分子修饰，并分离鉴定出5 个E G C G 甲基化衍生物，分别为4 ″-Me-EGCG 、 4 ′,4 ″-di-Me-EGCG 、5,3 ′,4 ′,5 ′,3 ″,4 ″,5 ″-hepta-Me-EGCG 、5,7,3 ′,4 ′,3 ″,4 ″,5 ″-hepta-Me-EGCG 、5,7,3 ′,4 ′,5 ′, 3 ″,4 ″,5 ″-octa-Me-EGCG 。 关键词：表没食子儿茶素没食子酸酯；甲基化；分子修饰；碘甲烷；化学合成 Methylation of (-)-Epigallocatechin-3-gallate (EGCG) . . LU Hai-pen g ，SUN Ye-lian g ，LIN Zhi * ，TAN Jun -fen g ，GUO Li (Engineering Research Center for Tea Processing, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 3 10008, China) Abstract ：For EGCG methylation via chemosynthesis, methyl iodide was used as methyl donor and two reaction systems (No.1 and No.2) were designed, in which the molar ratios between EGCG and methyl iodide were set at 1:80 and 1:1, respectively. Reaction products TRI-1 and TRI-2 obtained from reaction system No.1 and No.2, respectively were characterized by HPLC- MS and NMR. The results showed that the molecular modification of EGCG could be successfully accomplished by the use of chemosynthesis, and five EGCG derivatives, namely 4 ″-Me-EGCG, 4 ′,4 ″-di-Me-EGCG, 5,3 ′,4 ′,5 ′,3 ″,4 ″,5 ″-hepta- Me- EGCG, 5,7,3 ′,4 ′,3 ″,4 ″,5 ″-hepta- Me-EGCG and 5,7,3 ′,4 ′,5 ′,3 ″,4 ″,5 ″-octa- Me-EGCG were identified in the two reaction products, of which, 3 were contained in reaction product TRI- 1 and 2 in reaction product TRI-2. Key words ：EGCG ；methylation ；molecular modif