Chapter 9, part B.ppt

Chapter 9, part B Biotechnology and Recombinant DNA Genetic Engineering Genetic Engineering Making a Gene Product Making a Gene Product E. coli Used because it is easily grown and its genomics are known Need to eliminate endotoxin from products Cells must be lysed to get product Making a Gene Product Therapeutic Applications Subunit vaccines Nonpathogenic viruses carrying genes for pathogens antigens as vaccines Gene therapy to replace defective or missing genes Human Genome Project Nucleotides have been sequenced Human Proteome Project may provide diagnostics and treatments Random Shotgun Sequencing Scientific Applications Understanding of DNA Sequencing organisms genomes DNA fingerprinting for identification Southern Blotting Southern Blotting Southern Blotting Agricultural Applications Genetic Engineering Using Agrobacterium Safety Issues and Ethics Avoid accidental release Genetically modified crops must be safe for consumption and for the environment Who will have access to an individuals genetic information? * Copyright ? 2004 Pearson Education, Inc., publishing as Benjamin Cummings Copyright ? 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint? Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA ? FUNKE ? CASE Figure 9.11.1 Figure 9.11.2 Figure 9.12.1 Figure 9.12.2 Figure 9.13 Figure 9.14 Figure 9.16 Figure 9.15.1 Figure 9.15.2 Figure 9.15.3 Table 9.2 Figure 9.18 * Copyright ? 2004 Pearson Education, Inc., publishing as Benjamin Cummings