基于比较基因组学玉米ESTs定位方法.pdfVIP

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基于比较基因组学玉米ESTs定位方法.pdf

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ESTs∗ 1,2 1,2 2 1 2 ( , 071001; , 430070) ESTs ESTs 139 ESTs 96 ESTs 69%77 ESTs 55% ESTs A strategy based on comparative genomics to align ESTs of maize Zuxin Zhang1,2 Shaopeng Zhang1,2 Yonglian Zheng2 (1 College of Agronomy,Hebei Agricultural University, Hubei Baoding 071001;2 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hubei Wuhan 430070) Abstract: In this study, a new strategy to locate ESTs on maize linkage groups was described. In the strategy, the genomic sequence database of rice was employed to locate maize EST on rice linkage groups, and then to locate on maize linkage group by comparative genetics mapping between rice and maize genome. The aligned ESTs information should available for further study on genomics and gene cloning. As an example, 139 ESTs of maize were assayed, and 96 maize ESTs 69%were homologous with rice genomic sequence, 55% (77/139) ESTs were located on maize linkage groups based on the strategy, indicating that the locating approach of ESTs is feasible and available. Key words: Expression sequence tags (ESTs); Comparative mapping; genome; Molecular marker ESTsESTs ESTsBAC FISHESTs ESTsESTs ESTsESTs ESTsin silicoESTs [1]ESTsESTs ESTs 2 [2-5]Ahn1993RFLP ∗ “”9482003 Q03-1-22 Tel 0312-7528412 e-mail zux_zhang@263.net [6] QTL [7]