木薯基因组SSR与EST-SSR在麻疯树与橡胶树中的通用性分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2011, 37(1): 7478 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.00074 木薯基因组SSR 和EST-SSR 在麻疯树和橡胶树中的通用性分析 文明富 陈 新 王海燕 卢 诚 王文泉* , 571101 : 419 EST-SSR 182 SSR 5 2 , EST-SSR 55.85% 38.90%, SSR 37.36%26.37% , EST-SSR SSR, EST-SSR SSR SSR QTL : ; ; ; EST-SSR; SSR Transferability Analysis of Cassava EST-SSR and Genomic-SSR Markers in Jatropha and Rubber Tree * WEN Ming-Fu, CHEN Xin, WANG Hai-Yan, LU Cheng, and WANG Wen-Quan Institute of Tropical Bioscience Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China Abstract: Euphorbiaceae family includes abundant economic species, such as rubber tree, cassava, castor bean, and Jatropha. Cassava (Manihot esculenta Crantz) ranks in the sixth food crop in the world. In China, cassava is also an important tropical eco- nomic crop. The genomic-SSRs derived from cassava genome, and EST-SSRs derived from expressed sequence tags (ESTs). In this study, the transferability of 419 pairs of EST-SSR primer and 182 pairs of genomic-SSR primer from cassava was tested in five Jatropha lines and two rubber tree lines. The results showed that the transferability rate of cassava EST-SSR in Jatropha and rubber tree was 55.85% and 38.90%, and the transferability rate of cassava genomic-SSR in Jatropha and rubber tree was 37.36% and 26.37%, respectively. The transferability EST-SSR was higher for cssava than that of genomic-SSR. Besides, the transferability of cassava EST-SSR and genomic-SSR was higher in Jatropha than in rubber tree. These results suggested that the cassava SSR can be used for comparative mapping, gene