白藜芦醇对培养大鼠脑皮层星形胶质细胞增殖和谷氨酸代谢的影响.pdfVIP

中国现代医药杂志 年 月第 卷第 期 · · 2009 1 11 1 MMJC, Jan 2009, Vol 11, No．1 56 白藜芦醇对培养大鼠脑皮层星形胶质细胞增殖 及谷氨酸代谢的影响 郑小华 曹颖平 侯娟 王梅华 郑培烝 【摘要】 目的 探讨不同浓度白藜芦醇 ， 对培养大鼠脑皮层星形胶质细胞增殖及谷氨酸代谢功能的影 (resveratrol Res) 响。 方法 原代提取并纯化培养脑皮层星形胶质细胞。 采用 溴脱氧尿核苷（ ）掺入法检测细胞增殖；通过同位素掺 5- BrdU 入法和 ELISA 法检测谷氨酸代谢指标：谷氨酸摄取量、总谷胱苷肽（GSH ）含量和谷氨酰胺合成酶（Glutamine Synthetase, GS ）含量与活性。结果 Res 抑制细胞增殖并随着浓度升高和培养时间延长而抑制增强。Res 浓度为25μM 和50μM 时，谷 氨酸摄取量和GSH 含量显著增加（P0.05 ）。 而大于250μM 时，两者却降低，但在此高浓度下，GS 的细胞含量与活性却显 著升高（P0.05 ），而在低浓度时无任何变化。 结论 Res 可抑制星形胶质细胞增殖；对其谷氨酸代谢具有二元效应，即低浓 度可促进谷氨酸代谢，高浓度则降低谷氨酸代谢。 【关键词】 白藜芦醇 星形胶质细胞 谷氨酸代谢 The effect of resveratrol on the cell proliferation and the function of glutamate metabolism in primary cortical astro- ， ， ， cytes cultures of rats Zheng Xiaohua Cao Yingping Hou Juan et al.The Affiliated Union Hospital of Fujian Medical Univer- sity,Fuzhou 350001 【Abstract 】 Objective To investigate the effect of different concentrations of resveratrol on the cell proliferation and the function of glutamate metabolism in primary cortical astrocytes of rats. Methods Astrocyte cultures were prepared of cerebral cortex from neonate SD rats. The proliferation was measured by the incorporation of BrdU; Glutamate uptake was measured us- 3 ing H －glutamate;the activity of glutamine synthetase and content of glutathione were measured by enzymatic colorimetric assays; the content of glutamine synthetase was measured by ELISA. Results The proliferation was decreased,and followed by the con- centration and the time.At concentration of 25μM and 50μM, resveratrol was able to increase glutamate uptake and glutathione