仿土蛋鸡群J亚型禽白血病病毒感染状态评估及其来源探究.pdfVIP

第二届全国禽病分子生物技术青年学者论坛论文集 仿土蛋鸡群J亚型禽白血病病毒的感染状态评估及其来源探究 张培培1，刘绍琼，王健，王波，赵成棣，崔治中，孙淑红。 (山东农业大学动物医学院，泰安271018) 摘要：对山东某鸡场饲养的仿土蛋鸡进行J业型禽白血病病毒(ALV-J)的感染状态评估并探究其来 源。该鸡场鸡只消瘦，羽毛蓬乱，生长迟缓，表现出疑似禽白血病等免疫抑制病感染后的皿临床症 10d后用IFA试验进行ALV-J的鉴定，阳性率为100％(6／6)：跟踪该鸡群争115H龄调查其产蛋性 能，发现整个鸡群超过1lOIl龄的开产LJ期均／fi产蛋，随机剖检10只母鸡，均表现为胸腺、法氏囊 萎缩，卵巢朱发育，腺胃乳头出血。同时，提取4份来自仿土蛋鸡和2份来自其父本HR土公鸡的 性最高达99．7％。该结果表明该仿土蛋鸡群中ALV-J可能来源于其父本HR土公鸡。 关键词：仿土蛋鸡：J亚型．禽EJ血病病毒：感染状态：遗传变异；来源探究 InfectionStatusEvaluationandSource of JAvianLeukosisVirus ExplorationSubgroup inImitated Layers Free-range ZHANG LIU WANG-boZHAO CUI Pei··pei WANO-jian Cheng-diZhi·zhong Shao··qiong SUN Shu．hong* Medicine 271018,China) (CollegeofVeterianryofShandongAgriculturalUniversity,Taian JAvianleukosis infect recent in Abstract：Subgroupvirus(ALV-J)commonlylayers yearsChina．11hey are foreconomiclossesduetoboth and inchickens．The responsible mortalitydepressedperformance imitated oldwith was to of45 feather and skinny free—rangelayers days unkemptgrowedslowly suspected infectALVandother diseases．Inordertoestimatetheinfectionstatusand the immunosuppressive explore sourceofALV-Jinthe cloacalswabswerecollected forthemeasurementof flock，30 randomly p27 immunosorhent ratewas further antigenbyenzyme-linked assay(ELISA)，thepositive 87％(26／30)．For research，6 werecollected with