血管内皮生长因子-C在胃癌细胞株与胃癌组织中的表达及其临床意义、.docVIP

血管内皮生长因子-C在胃癌细胞株和胃癌组织中的表达及其临床意义、 　　作者：周慧聪,许培荣,张,艳,李继昌　　作者单位：新乡医学院第一附属医院消化内科，河南 卫辉 郑州大学实验中心，河南 郑州 郑州大学基础医学院药理学教研室，河南 郑州 郑州大学第一附属医院消化内科,河南 郑州 　　【摘要】目的 探讨血管内皮生长因子-C(VEGFC)在不同类型胃癌细胞株及组织中的表达及其与临床病理之间的关系。方法 应用反转录-聚合酶链反应(RTPCR)、Western blot分别检测VEGFC mRNA及其蛋白在胃癌细胞株中的表达，对53例胃癌及癌旁组织中VEGFC的表达采用免疫组织化学SP法染色检测，Ⅷ因子抗体及Ⅳ型胶原抗体双重免疫组织化学染色(SABC)法进行微淋巴管染色及微淋巴管计数。结果 在胃癌细胞株中均可检测到VEGFC mRNA及蛋白的表达，胃癌组织中，VEGFC蛋白的表达与淋巴结转移、病理分期有关(P0.05);淋巴管密度与VEGFC蛋白的表达、淋巴结转移、病理分期有关(P0.05)。结论 VEGFC表达与胃癌淋巴结转移密切相关，并有可能参与了胃癌淋巴管的形成。 　　【关键词】 胃癌;血管内皮生长因子-C;淋巴管;淋巴结转移 　　The role of vascular endothelial growth factorC and its expression in the gastric cancer tissues and cell lines ZHOU Huicong1，XU Peirong2，ZHANG Yang3，et al (1.Department of Gastroenterology ，the First Affiliated Hospital of Xinxiang Medical College，Weihui 453100，China;2.Experimental Center of Zhengzhou University，Zhengzhou 450052，China;3.Department of Pharmacology，College of Basic Medicine，Zhengzhou University,Zhengzhou 450052，China) 　　Abstract: Objective To study the relation between clinicopathological characteristic with the expression of vascular endothelial growth factor (VEGFC) in different type of gastric cancer cell lines.Methods The expression of VEGFC mRNA and protein in gastric carcinoma cell lines was examined by reverse transcriptionpolymerase chain reaction (RTPCR) and Western blot respectively.Fifty and three cases′ expression of VEGFC in gastric cancer and in the paracancer tissue were detected by immunohistochemical methods.Double immunohistostaining with Ⅷ factor antibody and Ⅳ collagen antibody was used to stain and count the microlymphatic.Results The expression of VEGFC mRNA and its protein in all gastric carcinoma cell lines could be detected.Expression of VEGFC in gastric cancer associated with lymphatic metastasis and pathological staging (P0.05).Lymphatic density also concerned with the expression of VEGFC,lymphatic metastasis and pathological staging(P0.05).Conclusion VEGFC may play an imp