生物有机肥对烤烟生长和相关防御性酶活性的影响_肖相政.pdfVIP

华北农学报 ·2 0 10, 2 5 (1):17 5 -17 9 1 1 2 1 1 肖相政,刘可星, 张志红,刘　亮,廖宗文 (1. , 　5 10642;2. , 　5 10642 ) 　　: FBOF1 FBOF2, (Greenfluorescenceprotein, GFP), , PPO、 PO、PAL、LOX 。, , 、、、, 30 d 26.20% 30.87%, , 30.60% 36.7 2%, 47.93% 23.97%;, , 、、 ;, ;, , 7 d, PPO 38.1% 35.8%, LOX 19.6% 17.5%, PAL 24.5%。 :;;; :S14 1　　:A　　:1000-7091(2010 )01-0 175-05 EffectofBiologicalOrganicFertilizeronFlue-curedTobacco Growthand efensive-relatedEnzymesActivity 1 1 2 1 1 XIAOXiang-zheng, LIUKe-xing, ZHANGZh-ihong, LIULiang, LIAOZong-wen (1.NewFertilizerResourcesCente,rSouthChinaAgriculturalUniversity, Guangzhou　510642, Chin;a 2. eparmtentofSoilScience,sSouthChinaAgriculturalUniversit,yGuangzhou　510642, China) Abstrac:tInthisarticlethemethodofgreenfluorescenceproteinandantibioticswereadoptedtomarkthespe- cificfunctionalmicrobesforresearchingtheeffectofbiologicorganicfertilizerFBOF1 andFBOF2 onflue-curedto- baccogrowthanditsdefensive-relatedenzyme,smeanwhil,edependingonthepotexpermientthecolonizationoffunc- tionalmicrobesintherhizospherewasresearched, andtheeffectofbiologicalorganicfertilizeronflue-curedtobacco growthandthedefensive-relatedenzymessuchaspolyphenoloxidas,eperoxidas,ephenylalanineammonialyaseandli- poxygenasewasalsoresearched.Theresultsindicatedthatutilizingbiologicalorganicfertilizercouldpromotetobacco growthandthebiomassindexesasplantheigh,tleafare,afreshweightanddryweightincreased.Theleafareaof30 th daymiproved26.20% and30.87% comparedwiththecontro,landthedifferenceisobviou,sandthefreshweightin- creased30.6% and36.72%, whilethedryweightis47.93% and23.97%morerespectively.Thesoilnutrientstatus wasalsomiprovedbyapplyingthebiologicalorganicfertilize