2007_2008年吉林省H3N2亚型流感病毒HA1基因序列分析.pdfVIP

2009 2 8 1 Chinese Journal of Public H ealth Engineering Feb 2009, V ol 8 No1 2007- 2008H3N2 HA 1 1 2 2 2 2 1 许爽, 吴东林 , 李响 , 沈博 , 杨显达 , 谭岩 ( 1吉林大学第 一医院中心实验室, 吉林长春 130021; 2吉林省疾病预防控制中心) 2007- 2008H 3N2 HA 1 MD- CK , RNA, 2007- 2008 H 3N2 989% ~ 100% , WHO A /W isconsin/67/0 10~ 14, 122; 11 12 ; 8 2007- 2008H 3N2 WHO A /W isconsin/67/0 ; ; H 3N2; ; R3732 A 1671-4199( 2009)01-0005-03 Analysis on Characteristics of HA1 Gene of Infl enzaH3N2 Isolated from J ilin P rovince d ring 2007- 2008 X U Sh uang, W U D ong - lin, LI X iang, et alJ ilin P rov inc ial Center f or D isease Con tro l and P reven tion, Chang chun J ilin 130062, Ch ina Abstract Objective To analyze the characteristics of HA1 gene of influenza H 3N2 isolated from Jilin province du ring 2007- 2008, and elucidate the relationsh ip betw een the influenza ep idem ic and the variation ofHA 1 region of the virusM ethods The virusesw ere isolated and propagated byMDCK cell RNA swere extracted and theirHA1 genesw ere amp lified by reverse transcription polym erase chain reaction ( RT - PCR) The purified PCR products were sequenced R es lts The pandem ic strains of influenzaH 3N2 isolated in Jilin province 2007- 2008 had 989% - 100% identity of nucleotides Comparedw ith A /W isconsin/67/0 vaccine strain recomm ended byWHO , 10- 14 am ino acid had changed, and therew as onem ore glycosylation site at 122nd site The virus isolated in Changchun city in Novem ber and Decem ber located in two different branches of the phylogenetic tree The influenza strains of eight regions form ed wt o clusters in the phylogenetic tree Concl sion Com pared w ith A /W isconsin/67/0 , The viruses of influenza H 3N2 in Jilin province 2007- 2008 occur evident antigen ic drift in HA1 region