天山冻土中嗜冷酵母菌生物多样性.pdfVIP

天山冻土中嗜冷酵母菌生物多样陛 姜远丽 ， 郑晓吉 ， 史学伟 ， 倪永清 ( 河予火学 食品学院，新疆 石河 子832000) 摘要：采用麦芽浸膏富集平板涂布天山冻土中可培养酵母茵，通过麦芽浸膏分离培养基筛选菌 株 。采用酵母菌常规生理生化实验、最适生长温度、最适pH对分离菌株的生理学进行研究 以 NL1和 NL4为PCR扩增 引物，通过 26SrRNA基 因D1／D2区序列同源性分析 ，初步确定酵母菌 株 的系统进化地位 。分 离筛选到 4株嗜低 温酵母 菌 ，分属于隐球酵母 (Cystofilobasidium infirmominiatum)、掷孢酵母 (Sporobolomycespatagonicus)、棒孢酵母 (Clayt’sporalusitaniae)和毕赤 酵-(Pichiakluyveri)。 关键词 ：天山冻土 ；嗜冷酵母菌；26SrDNA：多样性 中图分类号 ：TS261．11 文献标志码 ：A 文章编号 ：l673—1689(2012)12— 1289—06 DiversityofPsychr0tr0phicYeast om PermafrostSoilattheTerminusofa GlacierintheTianshanM ountains JIANGYuan—li， ZHENGXiao-ji， SHIXue—wei，NIYong-qing* (Foodcollege，ShiheziUniversity，Shihezi832000，China) Abstract：Theobjectiveofthisstudywastoinvestigatethediversityofcuhurableyeaststrainsfrom permafrostsoilsattheterminusofaglacierin theTianshanMountainswasinvestigated ． Isolati0n andmolecularphylogeneticanalysiswereperformed in ordertoexpandourknowledgeondiversity ofpsychrotrophicandpsychrophilicyeast．Inaddition，effortsfocusedonscreeningforpsychrophilic yeast．Psychrophilicyeaststrainsweredetectedon YEPG plates，respectively．Identityand genetic diversityofstrainsisolatedwasdeterminedby26SrRNA geneDl／D2bytheforwardprimersNL1 and the reverse NIA．The physiologicaltestswere carried outto determine the phonotypic differencesbetween strainsshowinghigh similarity of26S rRNA gene sequences ． In all，4 yeast stains was isolated by 26S rRNA gene D1／D2 and internaltranscribed spacers of4 genera (Cryptococcus，Sporobolomyces，Clayispora，Pichia)．The resultsenrich our knowledge on the differencesofpsychrotrophicyeastdiversitybetweentheglacier